ABSTRACT

The oxidative stress damage on cells is an example of roles in the pathogenesis of different degenerative diseases and the search of compounds that can slow this oxidation is continuous. The aim of this work was to obtain bioactive fractions from wild shrimp (Litopenaeus stylirostris) muscle, in order to evaluate their protective capacity and chemo-structurally characterize them. ABTS and DPPH, and FRAP assays suggested that bioactive fractions possess free radical-scavenging capacity, and reducing power, respectively. An inhibitory effect observed on AAPH-induced hemolysis and results from an H₂O₂-derived radicals-scavenging assay (retinoprotective) suggest that these fractions can exert their protective activity in human cells. UV–Vis, fluorescence, ¹³C-, ¹H-NMR, and ESI-MS studies performed on the most bioactive fraction, suggests that their main components are eicosapentaenoic acid, dioctyl phthalate, and a possibly novel indolocarbazole alkaloid derivative. These results suggest that these compounds are good candidates to further investigations as possible chemoprotective agents.

摘要

对细胞的氧化应激损伤是不同变性疾病的发病机理中作用的一个实例，并且可以减缓这种氧化作用的化合物的研究是连续的。这项工作的目的是从野虾（Litopenaeus stylirostris）肌肉中获得生物活性成分，以评估其保护能力并对其化学结构进行表征。ABTS和DPPH以及FRAP分析表明，生物活性成分分别具有清除自由基的能力和还原能力。观察到的对AAPH诱导的溶血的抑制作用以及H ₂ O ₂衍生的自由基清除试验（视黄醛保护性）的结果表明，这些级分可以在人细胞中发挥保护作用。UV-Vis，荧光，对最具生物活性的部分进行的¹³ C-，¹ H-NMR和ESI-MS研究表明，它们的主要成分是二十碳五烯酸，邻苯二甲酸二辛酯和可能是新颖的吲哚并咔唑生物碱衍生物。这些结果表明这些化合物作为可能的化学保护剂是进一步研究的良好候选者。