ABSTRACT Carbon steel is currently being considered as a candidate canister material for use in a deep geological repository of radioactive waste. Assessment of canister material corrosion through microbial activity is an important part of the safety assessment for the final repository. The aim of study was to compare and characterise the corrosion behaviour of carbon steel under sterile and non-sterile anaerobic conditions in natural groundwater containing sulphate-reducing bacteria (SRB). A molecular-biological approach was used to determine the presence and abundance changes of relevant bacterial groups. Carbon steel corrosion rates were higher in the presence of SRB compared with sterile control. EIS described the evolution of three time-constants under non-sterile conditions, while scanning electron microscopy confirmed that the carbon steel surface was covered with a two-layer biofilm. Molecular-biological analysis of the water and biofilm indicated the dominance of SRB, with Desulfomicrobium and Desulfovibrio species prevalent.

中文翻译：

---

在厌氧硫酸盐还原菌存在下对碳钢的微生物影响腐蚀

摘要 目前，碳钢被认为是用于放射性废物深层地质处置库的候选罐材料。通过微生物活动评估罐材料腐蚀是最终储存库安全评估的重要组成部分。研究的目的是比较和表征碳钢在无菌和非无菌厌氧条件下在含有硫酸盐还原菌 (SRB) 的天然地下水中的腐蚀行为。使用分子生物学方法来确定相关细菌群的存在和丰度变化。与无菌对照相比，存在 SRB 的碳钢腐蚀速率更高。EIS 描述了三个时间常数在非无菌条件下的演变，而扫描电子显微镜证实碳钢表面覆盖有两层生物膜。水和生物膜的分子生物学分析表明 SRB 占主导地位，其中 Desulfomicrobium 和 Desulfovibrio 物​​种普遍存在。