ABSTRACT The study was aimed at the migration and transformation of lead compounds in the rhizosphere, its accumulation in plants under the influence of the rhizosphere bacteria. For experiment, soil samples of the technogenous ecosystem contaminated differently by lead have been selected for plant growing. The samples were subdivided into control soil and the soil, inoculated by Azotobacter and Bacillus rhizobacteria. Lead concentrations have been analysed in easily exchangeable, carbonate, organic and Fe hydroxide-associated fractions as well in chelate forms and fulvic and humic acids. In soils, inoculated by rhizobacteria, there is an increased mobilisation of lead due to its decrease in humic acids and increase in fulvic acids. On technogenic soil, rhizobacteria initiate the immobilisation of Fe-hydroxide-bound, chelate-bound lead in the rhizosphere as well as lead occurring in roots. As a results, there is a decreased lead uptake by upper parts of plants. There is also a correlation between increasing soil alkalinity and increasing Pb accumulation in the roots of plants. The results of the experiment helped to understand more about the mechanisms of Pb compound behaviour under the influence of rhizobacteria that can be used for developing biotechnologies related to soil bioremediation and crop production.

中文翻译：

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芽孢杆菌和根际固氮菌影响下土壤-植物系统中先导化合物的转化

摘要 本研究旨在研究先导化合物在根际的迁移和转化，以及在根际细菌的影响下其在植物中的积累。为了进行实验，选择了被铅不同污染的技术生态系统的土壤样品用于植物生长。将样品细分为对照土壤和接种固氮菌和根际芽孢杆菌的土壤。已对易交换、碳酸盐、有机和氢氧化铁相关馏分以及螯合物形式、富里酸和腐殖酸中的铅浓度进行了分析。在被根际细菌接种的土壤中，由于腐殖酸减少和富里酸增加，铅的迁移增加。在技​​术土壤上，根际细菌启动了与氢氧化铁结合的固定化，根际中的螯合铅以及根中的铅。结果，植物上部对铅的吸收减少。土壤碱度增加与植物根部铅积累增加之间也存在相关性。实验结果有助于更多地了解根际细菌影响下铅化合物行为的机制，可用于开发与土壤生物修复和作物生产相关的生物技术。