The combination of genetic and phenotypic characters for species delimitation has allowed the discovery of many undescribed species of Neotropical amphibians. In this study, we used DNA sequences (genes 12S, 16S, ND1 and COI) and morphologic, bioacoustic and environmental characters of the Boana semilineata group to evaluate their phylogenetic relationships and assess their species limits. In addition, we included DNA sequences of several species of Boana to explore cryptic diversity in other groups. We found three Confirmed Candidate Species (CCS) within the B. semilineata group. Holotype examination of Hyla appendiculata shows that it is a valid species that corresponds to one of the CCS, which is here transferred to Boana. We describe the two remaining CCS. Our phylogeny highlights a number of secondary but meaningful observations that deserve further investigation: (1) populations of B. pellucens from northern Ecuador are more closely related to B. rufitela from Panama than to other Ecuadorian populations of B. pellucens; (2) we report, for the first time, the phylogenetic relationships of B. rubracyla showing that it is closely related to B. rufitela and B. pellucens; and (3) B. cinerascens and B. punctata form two species complexes consisting of several unnamed highly divergent lineages. Each of these lineages likely represents an undescribed species.

中文翻译：

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Boana semilineata物种组（Anura：Hylidae）的系统学，描述了来自亚马逊厄瓜多尔的两个新物种

遗传和表型特征的组合为物种划界提供了许多未描述的新热带两栖动物物种的发现。在这项研究中，我们使用了Boana semilineata组的DNA序列（基因12S，16S，ND1和COI）以及形态，生物声学和环境特征来评估它们的系统发生关系并评估其物种限制。此外，我们包括了几种蟒蛇的DNA序列，以探索其他群体中的神秘多样性。我们在B. semilineata组中找到了三个确认的候选物种（CCS）。盲HyHyla的全型检查表明，它是与CCS之一相对应的有效物种，CCS在这里已转移至Boana。我们描述了剩下的两个CCS。我们的系统进化论突出了许多次要的但有意义的观察结果，值得进一步研究：（1）来自厄瓜多尔北部的B. pellucens种群与来自巴拿马的rufitela B.亲缘关系比其他厄瓜多尔的B. pellucens种群更为紧密；（2）我们首次报道了红景天芽孢杆菌的系统发育关系，表明它与鲁菲特芽孢杆菌和血缘芽孢杆菌密切相关。（3）灰质芽孢杆菌和点状芽孢杆菌形成两个物种复合体，由几个未命名的高度趋异谱系组成。这些血统中的每一个都可能代表一个未描述的物种。B. rubracyla的系统发育关系表明它与rufitela B.和pellucens B.密切相关；（3）灰质芽孢杆菌和点状芽孢杆菌形成两个物种复合体，由几个未命名的高度趋异谱系组成。这些血统中的每一个都可能代表一个未描述的物种。B. rubracyla的系统发育关系表明它与rufitela B.和pellucens B.密切相关；（3）灰质芽孢杆菌和点状芽孢杆菌形成两个物种复合体，由几个未命名的高度趋异谱系组成。这些血统中的每一个都可能代表一个未描述的物种。