Studies on the long-term survival of animals often focus on the specific instance of survival of animals only, and descriptions of subsequent reproduction are generally not reported. In this study, we recorded the reproductive performance of the first-generation offspring of the resuscitated individual (SB-1) and the hatchling of the resuscitated egg (SB-3) of the Antarctic tardigrade, Acutuncus antarcticus, after being frozen for 30.5 years. By providing further detailed description of the reproduction of SB-1 and SB-3 after revival, and then comparing the reproductive performance with that of their first-generation offspring, the possible indications of the damage accrued during the long-term preservation in SB-1 and SB-3 were more specifically detected. Additionally, the DNA analysis revealed two distinctively different mitochondrial genetic sequences of A. antarcticus between the SB strains and the LSW strain. The observed differences in some of the reproductive parameters between the two genetic types suggested a possible relationship between the life-history traits and genetic type in the species A. antarcticus. Further experiments using the SB-1 and SB-3 strains reared for a long period to exclude the instant effect of preservation are expected to improve our understanding of the mechanisms underlying the long-term survival of animals.

中文翻译：

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冷冻超过30年后，南极的节肢动物Acutuncus antarcticus（Eutardigrada：Hypsibiidae）的繁殖性能得以恢复

关于动物长期存活的研究通常只关注动物存活的特定情况，通常不报告对随后繁殖的描述。在这项研究中，我们记录了复苏的个体的第一代后代（SB-1）的繁殖性能和南极缓行动物Acutuncus antarcticus的复苏的卵孵化（SB-3）的孵化，冻结30.5年后。通过提供复兴后SB-1和SB-3繁殖的进一步详细描述，然后将其繁殖性能与其第一代后代进行比较，可能表明在SB-和SB-3中长期保存期间会造成损害更具体地检测到图1和SB-3。此外，DNA分析揭示了SB菌株和LSW菌株之间南极拟南芥的两个不同的线粒体遗传序列。在两种遗传类型之间观察到的某些生殖参数差异表明，南极物种生活史特征与遗传类型之间可能存在关系。。预期使用长时间饲养的SB-1和SB-3菌株进行进一步实验，以排除保存的即时影响，有望增进我们对动物长期存活的潜在机制的了解。