Thulinius ruffoi is a freshwater species that has the ability to reproduce via parthenogenesis. A meroistic polytrophic ovary is present in this species. Analyses of the germarium structure, and formation and organization of female germ-cell clusters were performed using light, confocal laser scanning, transmission electron and serial block-face scanning electron microscopy. The germarium is the small, anterior part of an ovary that contains putative germ-line stem cells. In the studied species, the female germ-cell clusters are large and branched. Only one cell in each cluster develops into an oocyte, while all the other cells become trophocytes. In this paper, we present the first report on the presence of F-actin as a component of the intercellular bridges that connect the cells in the germ-cell cluster of T. ruffoi. Moreover, our results show that the female germ-cell clusters are formed as the result of both synchronous and asynchronous divisions and that their organization can vary not only between individuals of the investigated species, but also that clusters developing simultaneously within the same ovary can have a different spatial organization.

中文翻译：

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Thulinius ruffoi中的细菌和女性生殖细胞簇的结构（Bertolani，1982）（Tardigrada：Eutardigrada：Parachela）

Thulinius ruffoi是一种淡水物种，具有通过孤雌生殖繁殖的能力。在这个物种中存在有丝状多养性卵巢。使用光镜，共聚焦激光扫描，透射电子和连续块面扫描电子显微镜对细菌的细菌结构，雌性生殖细胞簇的形成和组织进行了分析。胚芽是卵巢的小前部，包含推定的种系干细胞。在所研究的物种中，雌性生殖细胞簇很大且分支。每个簇中只有一个细胞发育成卵母细胞，而其他所有细胞都变成营养细胞。在本文中，我们提出了关于F-肌动蛋白作为连接T细胞生殖细胞簇中细胞的细胞间桥成分的第一个报告。。ruffoi。此外，我们的结果表明，雌性生殖细胞簇是同步和异步分裂的结果，而且它们的组织不仅在所研究物种的个体之间可能不同，而且在同一个卵巢内同时发育的簇可以具有不同的空间组织。