Borrelia burgdorferi (Bb), which is neurotropic, can attack the central nervous system (CNS), leading to the development of various neurologic symptoms. The pathogenesis of Lyme neuroborreliosis (LNB) remains poorly understood. Presently, there is a lack of knowledge of the changes in mRNA and proteins in the CNS following early disseminated Lyme disease. Explants from the frontal cortex of 3 rhesus brains were incubated with medium alone or with medium containing live Bb for 6, 12, or 24 hours. Then, we analyzed identified mRNA and proteins in the frontal cortex tissues, allowing for an in-depth view of the transcriptome and proteome for a macroscopic and unbiased understanding of early disseminated Lyme disease in the brain. Through bioinformatics analysis, a complex network of enriched pathways that were mobilized during the progression of Lyme spirochete infection was described. Furthermore, based on the analysis of omics data, translational regulation, glycosaminoglycan/proteoglycan-binding activity in colonization and dissemination to tissues, disease-associated genes, and synaptic function were enriched, which potentially play a role in pathogenesis during the interaction between frontal cortex tissues and spirochetes. These integrated omics results provide unbiased and comprehensive information for the further understanding of the molecular mechanisms of LNB.

中文翻译：

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整合的转录组和蛋白质组分析为活的伯氏疏螺旋体和恒河猴额叶皮质植体之间的相互作用提供了新的见解

嗜疏性伯氏疏螺旋体（Borrelia burgdorferi，Bb）可以攻击中枢神经系统（CNS），从而导致各种神经系统症状的发展。莱姆病神经硼化病（LNB）的发病机理仍知之甚少。目前，缺乏关于早期传播的莱姆病后中枢神经系统中mRNA和蛋白质变化的知识。将3个恒河猴大脑额皮质的外植体与单独的培养基或含有活Bb的培养基一起孵育持续6、12或24小时。然后，我们分析了额叶皮层组织中鉴定出的mRNA和蛋白质，从而可以深入了解转录组和蛋白质组，从而对大脑中早期传播的莱姆病产生了宏观而公正的理解。通过生物信息学分析，描述了在莱姆螺旋体感染过程中动员的丰富途径的复杂网络。此外，基于组学数据的分析，丰富了翻译调控，定居和向组织中传播的糖胺聚糖/蛋白聚糖结合活性，疾病相关基因和突触功能，这些可能在额叶皮层之间相互作用的发病机理中发挥作用。组织和螺旋体。