ABSTRACT

Genomic structural variations (SVs) promote the evolution of Saccharomyces cerevisiae, and play an important role in phenotypic diversities. Yeast genomic structures can be remodeled by design and bottom-up synthesis. The synthesis of yeast genome creates novel copy number variations (CNVs) and SVs and develops new strategies to discover gene functions. Further, an inducible evolution system SCRaMbLE, consisted of 3,932 loxPsym sites, was incorporated on synthetic yeast genome. SCRaMbLE enables genomic rearrangements at will and rapidly generates chromosomal number variations, and massive SVs under customized conditions. The impacts of genetic variations on phenotypes can be revealed by genome analysis and chromosome restructuring. Yeast genome synthesis and SCRaMbLE provide a new research paradigm to explore the genotypic mechanisms of phenotype diversities, and can be used to improve biological traits and optimize industrial chassis.

中文翻译：

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通过酵母基因组合成和诱导型进化发现基因组结构变异并进行基因分型

摘要

基因组结构变异（SVs）促进酿酒酵母的进化，并在表型多样性中发挥重要作用。酵母基因组结构可以通过设计和自下而上的合成进行重塑。酵母基因组的合成产生新的拷贝数变异（CNV）和SV，并开发出发现基因功能的新策略。此外，由3,932个loxPsym位点组成的诱导型进化系统SCRaMbLE被掺入了合成酵母基因组中。SCRaMbLE可以随意进行基因组重排，并在定制条件下快速生成染色体数目变异和大量SV。遗传变异对表型的影响可以通过基因组分析和染色体重组来揭示。