Lactobacillus salivarius A3iob was administered to productive colonies belonging to commercial apiaries of small beekeepers (around 30–50 hives each one), from four departments of the province of Jujuy (Argentina): Yala, Tilquiza, El Carmen, and Los Alisos. The incidence of Varroa destructor and Nosema spp., before and after winter, was monitored during 2 years of study (2014–2015). Depending on the geographical location of each apiary and the application time, a monthly dose of the bacteria (10⁵ CFU/mL) reduced the levels of varroasis between 50 and 80%. Interestingly, L. salivarius A3iob cells remitted the percentage of the mites to undetectable values in an apiary treated with flumethrin (at Yala, Yungas region).

On the other hand, the spore levels of Nosema spp. in the lactobacilli-treated colonies also depended on the apiary and the year of application, but a significant decrease was mainly observed in the post-winter period. However, at Rivera (El Carmen’s department), no significant changes were detected in both parameters.

These results obtained after 2 years of work suggest that delivering L. salivarius A3iob cells to the bee colonies can become a new eco-friendly tool to cooperate with the control of these bees’ pests.

唾液乳杆菌A3iob被施用于来自胡胡伊省（阿根廷）的四个部门的小型养蜂人的商业养蜂场（每个蜂箱约30至50个蜂箱）：亚拉，蒂尔基萨，萨尔瓦多卡门和洛斯阿里索斯。在为期2年的研究（2014-2015年）中，监测了冬季前后Varroa破坏者和Nosema spp。的发生率。根据每个养蜂场的地理位置和使用时间，每月一次的细菌剂量（10 ⁵  CFU / mL）可以减少50％至80％的静脉痉挛。有趣的是，唾液乳杆菌A3iob细胞使螨虫的百分率达到了用氟氰菊酯处理过的蜂房（在Yungas地区的Yala）无法检测到的值。

另一方面，Nosema spp的孢子水平。经乳杆菌治疗的菌落的数量也取决于养蜂场和施用年份，但显着下降的主要原因是在冬季后。但是，在里维拉（El Carmen的部门），两个参数均未检测到显着变化。

经过2年的工作后获得的这些结果表明，将唾液乳杆菌A3iob细胞递送至蜂群可以成为与这些蜂害虫防治合作的新型生态友好工具。