Objective and design

Estrogen is one of the important regulators of the balance between bone formation and bone resorption that can modulate the levels and activity of certain growth factors and cytokines. In this study, we investigated the effect of 17β-estradiol (ED) on bone marrow (BM) cell differentiation in vivo and ex vivo in a mouse model of collagenase-induced osteoarthritis (CIOA).

Subject

ICR (CD-2) female mice were used in present experiments (total number = 75) and bone marrow cells were used for in vitro studies.

Treatment

Mice were orally fed under different schemes with 17β-estradiol at a dose of 2 μg or 4 μg for 30 days.

Methods

The effect of estradiol was estimated by histopathological, flow cytometry, and ELISA assays. Statistical differences were determined by one-way ANOVA.

Results

Estradiol treatment ameliorated cartilage destruction and osteophyte formation if started from day 0 of CIOA induction, attended with a decrease of uterine and ovarian weights. Long time treatment lowered the percentage of megakaryocyte/platelet (CD62P+) populations and osteoclast (RANK+) populations in BM. Cells obtained from estradiol-treated CIOA mice showed inhibited capacity to differentiate into RANK+ and mesenchymal cells under osteoclastogenic conditions in vitro. Estrogen decreased serum IL-6 levels.

Conclusion

Results indicate a potential protective role for estrogen against the development of OA.

中文翻译：

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雌二醇治疗对胶原酶诱导的关节炎骨髓细胞分化的影响

目标和设计

雌激素是骨形成与骨吸收之间平衡的重要调节剂之一，可以调节某些生长因子和细胞因子的水平和活性。在这项研究中，我们在胶原酶诱导的骨关节炎（CIOA）小鼠模型中研究了17β-雌二醇（ED）对体内和离体骨髓（BM）细胞分化的影响。

学科

ICR（CD-2）雌性小鼠用于本实验（总数= 75），骨髓细胞用于体外研究。

治疗

在不同方案下以2μg或4μg的剂量对小鼠口服17β-雌二醇，持续30天。

方法

雌二醇的作用通过组织病理学，流式细胞仪和ELISA分析进行评估。统计差异通过单向方差分析确定。

结果

如果从CIOA诱导的第0天开始，雌二醇治疗可改善软骨破坏和骨赘形成，并伴有子宫和卵巢重量的减少。长时间的治疗降低了BM中巨核细胞/血小板（CD62P +）和破骨细胞（RANK +）种群的百分比。在体外破骨细胞条件下，从雌二醇处理的CIOA小鼠获得的细胞显示出抑制分化为RANK +和间充质细胞的能力。雌激素降低血清IL-6水平。

结论

结果表明，雌激素对OA的发展具有潜在的保护作用。