Abstract

Several in vivo immunotropic effects of κ/β-carrageenan isolated from the red algae Tichocarpus crinitus were studied, by orally administering it at 100 mg/kg/day to mice for 7 days. Serum levels of IFN-γ, IL-12, IL-1β, and IL-4 were measured. Carrageenan’s ability to influence development of LPS-induced inflammation was also assessed. Oral administration of κ/β-carrageenan increased serum levels of all the studied cytokines at least twice in comparison to the intact mice, while intraperitoneal LPS injection at 1 mg/kg increased concentration of only the pro-inflammatory cytokines: IFN-γ, IL-12, and IL-1β. Furthermore, κ/β-carrageenan demonstrated a higher efficacy at inducing IFN-γ production than LPS. Previous 7-day-long oral carrageenan administration impaired development of LPS-induced inflammation: level of IL-1β dropped below that found in intact mice, while IFN-γ and IL-12 concentrations were at least 40% lower than in mice with LPS-induced inflammation. Murine peritoneal macrophages were also affected by the oral administration of the κ/β-carrageenan: their motility was increased, and morphology altered. In sum, we have demonstrated that κ/β-carrageenan, when administered orally, is not only not immunologically inert, but at the dose of 100 mg/kg possesses pharmacologically exploitable effects.

中文翻译：

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Tichocarpus crinitus 角叉菜胶的免疫调节特性。

摘要

从红藻Tichocarpus crinitus中分离出的 κ/β-角叉菜胶的几种体内免疫作用通过以 100 mg/kg/天的剂量对小鼠口服给药 7 天，进行了研究。测量了 IFN-γ、IL-12、IL-1β 和 IL-4 的血清水平。还评估了角叉菜胶影响 LPS 诱导的炎症发展的能力。与完整小鼠相比，口服 κ/β-角叉菜胶使所有研究的细胞因子的血清水平增加至少两倍，而腹腔内注射 1 mg/kg 的 LPS 仅增加促炎细胞因子的浓度：IFN-γ、IL -12 和 IL-1β。此外，κ/β-角叉菜胶在诱导 IFN-γ 产生方面表现出比 LPS 更高的功效。以前 7 天的口服角叉菜胶给药会损害 LPS 引起的炎症的发展：IL-1β 水平下降到低于完整小鼠的水平，而 IFN-γ 和 IL-12 的浓度至少比 LPS 诱导的炎症小鼠低 40%。小鼠腹腔巨噬细胞也受到口服 κ/β-角叉菜胶的影响：它们的运动性增加，形态改变。总之，我们已经证明，当口服给药时，κ/β-角叉菜胶不仅没有免疫惰性，而且在 100 mg/kg 的剂量下具有药理学可利用的作用。