Abstract

Esculetin, a coumarin derivative from various natural plants, has an anti-inflammatory property. In the present study, we examined if esculetin has any salutary effects against lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Acute lung injury (ALI) was induced via the intratracheal administration of LPS, and esculetin (20 and 40 mg/kg) was given intraperitoneally 30 min before LPS challenge. After 6 h of LPS administration, lung tissues were collected for analysis. Pretreatment with esculetin significantly attenuated histopathological changes, inflammatory cell infiltration, and production of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, in the lung tissue. Furthermore, esculetin inhibited the protein kinase B (AKT), extracellular signal-regulated kinase (ERK), and nuclear factor-kappa B (NF-κB) pathways and downregulated the expression of RORγt and IL-17 in LPS-induced ALI. Our results indicated that esculetin possesses anti-inflammatory and protective effects against LPS-induced ALI via inhibition of the AKT/ERK/NF-κB and RORγt/IL-17 pathways.

中文翻译：

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Esculetin通过调节AKT / ERK /NF-κB和RORγt/ IL-17途径改善脂多糖诱导的小鼠急性肺损伤。

摘要

Esculetin是多种天然植物的香豆素衍生物，具有抗炎特性。在本研究中，我们检查了七叶皂素是否对脂多糖（LPS）诱导的小鼠急性肺损伤（ALI）有任何有益作用。急性肺损伤（ALI）通过气管内给予LPS，在LPS攻击前30分钟腹膜内给予七叶内酯（20和40 mg / kg）。LPS给药6小时后，收集肺组织进行分析。七叶皂甙预处理可显着减轻肺组织中的组织病理学变化，炎性细胞浸润以及促炎性细胞因子的生成，例如肿瘤坏死因子（TNF）-α，白介素（IL）-1β和IL-6。此外，七叶亭抑制蛋白激酶B（AKT），细胞外信号调节激酶（ERK）和核因子-κB（NF-κB）通路，并下调LPS诱导的ALI中RORγt和IL-17的表达。我们的结果表明，七叶皂甙具有通过LPS诱导的ALI的抗炎和保护作用。 抑制AKT / ERK /NF-κB和RORγt/ IL-17途径。