The Antarctic krill, Euphausia superba, is a Southern Ocean endemic species of proven ecological importance to the region. In the context of predicted global warming, it is particularly important to understand how classic biomarkers of heat stress function in this species. In this respect, Hsp70s are acknowledged as good candidates. However, previous studies of expression kinetics have not been able to demonstrate significant upregulation of these genes in response to heat shocks at 3 °C and 6 °C for 3 and 6 h. The current work complements these previous results and broadens the prospects for the use of Hsp70s as a relevant marker of thermal shock in this krill species. New experiments demonstrate that induction of Hsp70 isoforms was not detected during exposure to heat shock, but increased expression was observed after several hours of recovery. To complete the analysis of the expression kinetics of the different isoforms, experiments were carried out over short time scales (1 and 2 h at 3 °C and 6 °C) as well as at higher temperatures (9 °C, 12 °C, and 15 °C for 3 h), without any significant response. A 6-week monitoring of animals at 3 °C showed that the time factor is decisive in the establishment of the response. CT_max experiments with incremental times of 1 °C per day or 1 °C every 3 days have shown a particularly high resilience of the animals. The demonstration of the abundance of Hsp70s present before thermal stress in various species of krill, as well as in specimens of E. superba of various origins, showed that the delay in the response in expression could be related to the high constitutive levels of Hsp70 available before the stress experiments. The alternative labelling of the two main isoforms of Hsp70 according to the origin of the animals allowed hypotheses to be put forward on the functioning of thermoregulation in Antarctic krill as well as ice krill.

中文翻译：

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变暖海洋中的南极磷虾（南极磷虾）：耐热性和破译 Hsp70 反应。


南极磷虾，南极磷虾，是南大洋特有物种，已被证明对该地区具有重要的生态重要性。在预测全球变暖的背景下，了解热应激的经典生物标志物如何在该物种中发挥作用尤为重要。在这方面，Hsp70 被认为是良好的候选者。然而，之前的表达动力学研究未能证明这些基因在 3°C 和 6°C 3 小时和 6 小时的热激反应中显着上调。目前的工作补充了这些先前的结果，并拓宽了使用 Hsp70 作为该磷虾物种热冲击相关标记的前景。新的实验表明，在热休克期间没有检测到 Hsp70 同工型的诱导，但在恢复几个小时后观察到表达增加。为了完成不同异构体的表达动力学分析，在短时间范围（3°C和6°C下1和2小时）以及更高温度（9°C、12°C、和 15°C 3 小时），没有任何明显的反应。在 3°C 下对动物进行为期 6 周的监测表明，时间因素对于建立反应具有决定性作用。每天增加 1°C 或每 3 天增加 1°C 的 CT _max实验表明，动物具有特别高的恢复能力。各种磷虾以及不同来源的木贼样本中在热应激之前存在的 Hsp70 丰度的证明表明，表达响应的延迟可能与可用的 Hsp70 的高组成水平有关。在压力实验之前。 根据动物起源对 Hsp70 的两种主要亚型进行替代标记，可以对南极磷虾和冰磷虾的体温调节功能提出假设。