The development of Portunus trituberculatus egg cells is directly related to the nutritional status of the fertilized egg, which affects the key production stages of offspring hatching. Vitellogenin plays a key role in the nutrient supply required for the development of the egg cells. The c-Jun N-terminal kinase (JNK) is an important member of the mitogen-activated protein kinase (MAPK) superfamily and plays an important role in cell proliferation, transformation, differentiation, and apoptosis. At present, there are no reports on the involvement of the JNK signaling pathway in the reproductive regulation of P. trituberculatus. In this study, rapid amplification of complementary DNA ends amplification technology was used to clone the full length of JNK complementary DNA, which has a length of 2094 bp, including an open reading frame (ORF) of 1266 bp encoding a 421-amino acid protein. The protein includes the S_TKC conserved domain with a TPY phosphorylation site, which is a typical feature of the JNK gene family. Observing tissue sections found the oocytes in the inhibitor group developed slowly, while the oocytes in the activated group showed accelerated development. Meanwhile, Portunus trituberculatus JNK and vitellogenin (Vg) genes exhibited the same trend in the hepatopancreas and ovaries, and the expression of the SP600125 group was downregulated (P < 0.05), while the anisomycin group was upregulated (P < 0.05). In addition, JNK enzyme activity and vitellin (Vn) content in the ovarian tissue showed that the JNK activity of the SP600125 group decreased, while activity increased in the anisomycin group. The accumulation of Vn content in the SP600125 group decreased, and that in the anisomycin group increased. In summary, after injection with inhibitor or activator, the JNK signaling pathway of P. trituberculatus was inhibited or activated, the accumulation of Vn in the ovary was reduced or increased, and ovarian development was inhibited or accelerated, respectively. These results indicated that the JNK signaling pathway is involved in the regulation of Vg synthesis and ovarian development in P. trituberculatus. The results of this study further add to the knowledge of the breeding biology of P. trituberculatus and provide a theoretical reference for the optimization of breeding techniques in aquaculture production systems.

中文翻译：

---

JNK信号通路调控梭子蟹卵巢的发育和卵黄蛋白原(Vg)的合成。


三疣梭子蟹卵细胞的发育与受精卵的营养状况直接相关，影响子代孵化的关键生产阶段。卵黄原蛋白在卵细胞发育所需的营养供应中发挥着关键作用。 c-Jun N端激酶（JNK）是丝裂原激活蛋白激酶（MAPK）超家族的重要成员，在细胞增殖、转化、分化和凋亡中发挥重要作用。目前尚未见JNK信号通路参与三疣梭子蟹生殖调控的报道。本研究采用互补DNA末端快速扩增扩增技术克隆了全长JNK互补DNA，其长度为2094bp，其中包含1266bp的开放阅读框（ORF），编码421个氨基酸的蛋白质。该蛋白包含带有 TPY 磷酸化位点的 S_TKC 保守结构域，这是 JNK 基因家族的典型特征。组织切片观察发现，抑制剂组卵母细胞发育缓慢，而激活组卵母细胞发育加速。同时，三疣梭子蟹JNK和卵黄蛋白原（Vg）基因在肝胰腺和卵巢中的表达趋势相同，SP600125组表达量下调（ P < 0.05），而茴香霉素组表达量上调（ P < 0.05）。此外，卵巢组织中JNK酶活性和卵黄蛋白（Vn）含量显示，SP600125组JNK活性降低，茴香霉素组活性升高。 SP600125组Vn含量积累减少，茴香霉素组Vn含量积累增加。综上所述，注射抑制剂或激活剂后，三疣梭子蟹的JNK信号通路受到抑制或激活，卵巢中Vn的积累减少或增加，卵巢发育受到抑制或加速。这些结果表明JNK信号通路参与了三疣梭子蟹Vg合成和卵巢发育的调控。本研究结果进一步丰富了三疣梭子蟹的育种生物学知识，为水产养殖生产系统中育种技术的优化提供了理论参考。