Centrifugal droplet-based microfluidic devices have been applied to biomedical analysis and diagnostics recently. However, in centrifugal droplet-based microfluidic devices, droplets are tightly packed (i.e., the oil film between neighbouring droplets is thin). Therefore, droplet coalescence usually occurs especially during thermal incubation process. To preserve individual droplets in the devices, we report a new design for monodisperse droplet generation and storage that exploits a centrifugal configuration for droplet emulsification and oil-storage structures (OSSs) for regulation of the thickness of oil film between neighbouring droplets. The centrifugal emulsifier was well designed to ensure uniform droplet generation. Meanwhile, the OSSs could store oil during centrifugal emulsification while release oil before thermal incubation, which “loosen” tightly packed droplets to prevent droplets from coalescing. In this paper, the working process of OSS was analysed, and its shape and size were optimized. Then, the optimized OSSs were integrated into a centrifugal emulsifier for droplet digital loop mediated isothermal amplification (ddLAMP) by which detection of JAK2 V617F mutation within myeloproliferative neoplasms with a dynamic range of 10¹ to 10⁴ copies per μL was achieved. We anticipate that the simplicity and robustness of our system make it attractive as an inexpensive and easy-to-operate device for DNA amplification, particularly applicable in point-of-care settings.

中文翻译：

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集成了储油结构的离心微流乳化机，用于坚固的数字LAMP

基于离心液滴的微流控设备最近已应用于生物医学分析和诊断。但是，在基于离心液滴的微流体装置中，液滴紧密堆积（即，相邻液滴之间的油膜很薄）。因此，液滴聚结通常发生，特别是在热培养过程中。为了将单个液滴保留在设备中，我们报告了单分散液滴产生和存储的新设计，该设计采用离心配置进行液滴乳化和储油结构（OSS），以调节相邻液滴之间的油膜厚度。离心乳化器经过精心设计，可确保产生均匀的液滴。同时，OSS可以在离心乳化过程中储存油，而在热孵化之前释放油，可以“松散”紧密堆积的液滴，以防止液滴聚结。本文分析了OSS的工作过程，并对其形状和尺寸进行了优化。然后，将优化的OSS集成到用于液滴数字环介导的等温扩增（ddLAMP）的离心乳化器中，通过该乳化剂检测动态范围为10的骨髓增生性肿瘤内的JAK2 V617F突变。每微升可实现¹至10 ⁴拷贝。我们预计，我们的系统的简单性和鲁棒性使其成为一种廉价且易于操作的DNA扩增设备，特别适用于即时医疗场所。