Three cholangiocarcinoma (CCA) cell line—formerly named, M156, M213 and M214 have been intensively used with discrepancy of their tumor origins. They were assumed to be originated from three different donors without authentication. To verify the origins of these cell lines, the short tandem repeat (STR) analysis of the currently used cell lines, the cell stocks from the establisher and the primary tumor of a CCA patient were performed. Their phenotypic and genotypic originality were compared. The currently used 3 CCA cell lines exhibited similar STR as CCA patient ID-M213 indicating the same origin of these cells. The cell stocks from the establisher, however, revealed the same STR of M213 and M214 cells, but not M156. The misidentification of M214 and M156 is probably due to the mislabeling and cross-contamination of M213 cells during culture. These currently used cell lines were renamed as KKU-213A, -213B and -213C, for the formerly M213, M214 and M156 cells, respectively. These cell lines were established from a male with an intrahepatic mass-forming CCA stage-4B. The tumor was an adenosquamous carcinoma with the liver fluke ova granuloma in evidence. All cell lines had positive CK19 with differential CA19-9 expression. They exhibited aneuploidy karyotypes, distinct cell morphology, cell growth, cytogenetic characteristic and progressive phenotypes. KKU-213C formed a adenosquamous carcinoma, whereas KKU-213A and KKU-213B formed poorly- and well-differentiated squamous cell carcinomas in xenografted mice. mRNA microarray revealed different expression profiles among these three cell lines. The three cell lines have unique characteristics and may resemble the heterogeneity of tumor origin.

中文翻译：

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来自 Opisthorchis viverrini 相关胆管癌患者的单个肿瘤的三种细胞系的功能和遗传特征。

三种胆管癌 (CCA) 细胞系——以前命名为 M156、M213 和 M214，已被广泛使用，但它们的肿瘤起源存在差异。他们被假定来自三个不同的捐赠者，未经认证。为了验证这些细胞系的来源，对当前使用的细胞系、建立者的细胞储备和 CCA 患者的原发性肿瘤进行了短串联重复 (STR) 分析。比较了它们的表型和基因型独创性。目前使用的 3 种 CCA 细胞系表现出与 CCA 患者 ID-M213 相似的 STR，表明这些细胞的来源相同。然而，建立者的细胞储备显示了相同的 M213 和 M214 细胞 STR，但不是 M156。M214和M156的误认可能是由于M213细胞在培养过程中贴错标签和交叉污染所致。这些目前使用的细胞系被重命名为 KKU-213A、-213B 和 -213C，分别用于以前的 M213、M214 和 M156 细胞。这些细胞系是从具有肝内肿块形成 CCA 4B 阶段的男性建立的。肿瘤是一种腺鳞癌，有肝吸虫卵肉芽肿的证据。所有细胞系均具有阳性CK19，具有差异CA19-9表达。它们表现出非整倍体核型、不同的细胞形态、细胞生长、细胞遗传学特征和进行性表型。KKU-213C 形成腺鳞癌，而 KKU-213A 和 KKU-213B 在异种移植小鼠中形成低分化和高分化鳞状细胞癌。mRNA 微阵列揭示了这三种细胞系之间的不同表达谱。