With global climate change in progress, a particular problem is that sudden drops in temperature affect the production of rice. In this study, we explored how to overcome the effects of chilling injury on rice seedlings. Wild-type Dular (WT) plants and Lsi1 (low silicon rice 1) overexpressing Dular plants (Lsi1-OX) were used as research materials. We found that Lsi1 enhanced the antioxidant system and non-structural carbohydrates (NSC) of rice at physiological levels. On this basis, Isobaric Tag for Relative Absolute Quantitation (iTRAQ) technology was used to examine the expression of the rice root proteins in response to low temperature stress. In addition, a total of 433 proteins were differentially expressed in this study, of which 284 were upregulated and 149 were downregulated. These proteins are divided into 24 functions. Moreover, our analysis found that the signaling pathway of the Lsi1-OX plant roots is significantly enhanced under low temperature stress, which in turn, led to the development of resistance reaction and transport pathways, indicating that Lsi1 increases the ability of rice to perceive and transmit low-temperature signals and stimulate the corresponding biochemical processes. In this study, we provided a theoretical basis to understand the mechanism related to the cold resistance of plants and therefor, accelerate the molecular breeding process of low temperature resistant crops.

中文翻译：

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Lsi1在提高水稻根系耐寒性中起积极作用

随着全球气候变化的进展，一个特别的问题是温度的突然下降会影响水稻的生产。在这项研究中，我们探讨了如何克服冷害对水稻幼苗的影响。野生型 Dular (WT) 植物和过表达 Dular 植物 (Lsi1-OX) 的 Lsi1 (低硅水稻 1) 被用作研究材料。我们发现 Lsi1 在生理水平上增强了水稻的抗氧化系统和非结构性碳水化合物 (NSC)。在此基础上，利用等压标签相对绝对定量（iTRAQ）技术检测水稻根系蛋白在低温胁迫下的表达情况。此外，本研究共有433种蛋白质差异表达，其中284种上调，149种下调。这些蛋白质分为24个功能。而且，我们的分析发现，Lsi1-OX 植物根系的信号通路在低温胁迫下显着增强，进而导致抗性反应和转运通路的发展，表明 Lsi1 增加了水稻对低温胁迫的感知和传递能力。 -温度信号并刺激相应的生化过程。本研究为了解植物抗寒性的相关机制，加快抗寒作物分子育种进程提供了理论依据。表明 Lsi1 增加了水稻感知和传递低温信号的能力，并刺激了相应的生化过程。本研究为了解植物抗寒性的相关机制，加快抗寒作物分子育种进程提供了理论依据。表明 Lsi1 增加了水稻感知和传递低温信号的能力，并刺激了相应的生化过程。本研究为了解植物抗寒性的相关机制，加快抗寒作物分子育种进程提供了理论依据。