The balance of pro-angiogenic and anti-angiogenic factors has a significant role in the development of diabetic cardiomyopathy. The purpose of this study was to examine the effect of 8 weeks of high-intensity interval training (HIIT) and moderate intensity continuous training (MICT) on the myocardial angiogenic factors and histological changes in male diabetic rats. Thirty-two male Wistar rats were randomly assigned into four groups: healthy non-exercised control, diabetic (D), D + HIIT, and D+ MICT groups. Diabetes type 2 was induced by a high-fat diet for 2 weeks and a single injection of streptozotocin. Following confirmation of diabetes, animals were subjected to HIIT (90 to 95% of VO2max) or MICT (50–65% of VO2max) protocols 5 days a week for 8 weeks. Western blotting was used for detection of protein expressions of vascular endothelial growth factor (VEGF), transforming growth factor-beta (TGF-β), matrix metalloproteinase-2 (MMP2), and tissue inhibitor of matrix metalloproteinase-2 (TIMP2) in the left ventricle. In addition, baseline and final blood glucose and body weight were measured. Histological changes were evaluated using H&E and Masson’s trichrome staining. The results showed that exercise increased protein levels of pro-angiogenic factors while reduced anti-angiogenic factors protein levels in diabetic animals. These changes were followed by increased capillary density and reduced interstitial fibrosis in the left ventricle. Moreover, the MICT was superior to HIIT in enhancing angiogenic factors and attenuation of blood glucose and fibrosis in the diabetic rats. These findings confirm the effectiveness of exercise, particularly MICT, in the improvement of diabetic cardiomyopathy.

中文翻译：

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8周高强度间歇训练和中强度连续训练对糖尿病雄性大鼠心脏血管新生因子的影响。

促血管生成和抗血管生成因子的平衡在糖尿病性心肌病的发展中具有重要作用。这项研究的目的是检查8周高强度间歇训练（HIIT）和中强度连续训练（MICT）对雄性糖尿病大鼠心肌血管生成因子和组织学变化的影响。将32只雄性Wistar大鼠随机分为四组：健康非运动对照组，糖尿病（D），D + HIIT和D + MICT组。高脂饮食2周和单次注射链脲佐菌素可诱发2型糖尿病。确认糖尿病后，动物每周5天接受HIIT（最大摄氧量的90％至95％）或MICT（最大摄氧量的50-65％）的方案，共8周。Western印迹用于检测血管内皮生长因子（VEGF），转化生长因子β（TGF-β），基质金属蛋白酶2（MMP2）和组织金属蛋白酶2组织抑制剂（TIMP2）的蛋白表达。左心室。此外，还测量了基线和最终血糖以及体重。使用H＆E和Masson三色染色评估组织学变化。结果显示，运动增加了糖尿病动物中促血管生成因子的蛋白质水平，同时降低了抗血管生成因子的蛋白质水平。这些变化之后，毛细血管密度增加，左心室间质纤维化减少。此外，在增强糖尿病大鼠的血管生成因子和减轻血糖及纤维化方面，MICT优于HIIT。