Bacillus subtilis is a remarkably diverse bacterial species that displays many ecological functions. Given its genomic diversity, the strain Bacillus subtilis EA-CB0575, isolated from the rhizosphere of a banana plant, was sequenced and assembled to determine the genomic potential associated with its plant growth promotion potential. The genome was sequenced by Illumina technology and assembled using Velvet 1.2.10, resulting in a whole genome of 4.09 Mb with 4332 genes. Genes involved in the production of indoles, siderophores, lipopeptides, volatile compounds, phytase, bacilibactin, and nitrogenase were predicted by gene annotation or by metabolic pathway prediction by RAST. These potential traits were determined using in vitro biochemical tests, finding that B. subtilis EA-CB0575 produces two families of lipopeptides (surfactin and fengycin), solubilizes phosphate, fixes nitrogen, and produces indole and siderophores compounds. Finally, strain EA-CB0575 increased 34.60% the total dry weight (TDW) of tomato plants with respect to non-inoculated plants at greenhouse level. These results suggest that the identification of strain-specific genes and predicted metabolic pathways might explain the strain potential to promote plant growth by several mechanisms of action, accelerating the development of plant biostimulants for sustainable agricultural.

中文翻译：

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枯草芽孢杆菌EA-CB0575基因组揭示了促进植物生长和可持续农业潜力的线索。

枯草芽孢杆菌是一种非常多样的细菌，具有许多生态功能。鉴于其基因组多样性，对从香蕉植物根际分离的枯草芽孢杆菌EA-CB0575菌株进行了测序和组装，以确定与其植物生长促进潜力相关的基因组潜力。通过Illumina技术对基因组进行测序，并使用Velvet 1.2.10进行组装，从而得到具有4332个基因的4.09 Mb完整基因组。通过基因注释或通过RAST的代谢途径预测来预测与吲哚，铁载体，脂肽，挥发性化合物，植酸酶，芽孢杆菌抑素和固氮酶的产生有关的基因。使用体外生化测试确定了这些潜在性状，发现枯草芽孢杆菌EA-CB0575产生脂肽的两个家族（表面活性素和丰霉素），增溶磷酸盐，固定氮，并产生吲哚和铁载体化合物。最后，相对于在温室水平的未接种植物，EA-CB0575菌株使番茄植物的总干重（TDW）增加了34.60％。这些结果表明，鉴定菌株特异性基因和预测的代谢途径可能解释了该菌株通过几种作用机制促进植物生长的潜力，从而加速了可持续农业植物生物刺激剂的开发。