Bidens pilosa is an herb popularly used to treat inflammation, hemorrhoids, fever, and gastric ulcers with reported pharmacological activities and chemical composition that sustain its selection as a potential intestinal anti-inflammatory product. Based on this, we examined the effects of a B. pilosa fatty acid-standardized supercritical preparation on the intestinal inflammatory process induced by trinitrobenzenesulphonic acid in rats, using either preventative or curative treatments. We also investigated the safety of plant extract by acute and sub-chronic toxicological analysis. The intestinal anti-inflammatory activity was related to modulation of the immune response, increasing IL-10 production and reducing IL-1β, IL-6, and TNF-α level, the oxidative stress, and the MUC production in the inflamed colon. Optic, scanning, and transmission electron microscopy (TEM) analysis supported the beneficial effects promoted by B. pilosa, which were closely related to downregulation of heparanase, Hsp70, Mapk 3, and NF-κB signaling and with the presence of long-chain fatty acids in extract. Our data suggest that B. pilosa supercritical preparation is a chemically standardized preparation potentially useful as complementary intestinal anti-inflammatory agent to treat inflammatory bowel disease.

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Bidens pilosa (Black Jack) 标准化提取物改善大鼠急性 TNBS 诱导的肠道炎症

Bidens pilosa 是一种广泛用于治疗炎症、痔疮、发烧和胃溃疡的草药，据报道其药理活性和化学成分使其被选为潜在的肠道抗炎产品。在此基础上，我们使用预防性或治疗性治疗方法检查了长毛双歧杆菌脂肪酸标准化超临界制剂对三硝基苯磺酸诱导的大鼠肠道炎症过程的影响。我们还通过急性和亚慢性毒理学分析研究了植物提取物的安全性。肠道抗炎活性与免疫反应的调节、增加 IL-10 的产生和降低 IL-1β、IL-6 和 TNF-α 水平、氧化应激和发炎结肠中 MUC 的产生有关。光学，扫描，和透射电子显微镜 (TEM) 分析支持由 B. pilosa 促进的有益作用，这与乙酰肝素酶、Hsp70、Mapk 3 和 NF-κB 信号传导的下调以及提取物中长链脂肪酸的存在密切相关。我们的数据表明 B. pilosa 超临界制剂是一种化学标准化制剂，可能用作补充肠道抗炎剂来治疗炎症性肠病。