Extrusion process using 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) in co-solvent, dimethyl sulfoxide (DMSO), was conducted to improve the pretreatment effect on the enzymatic saccharification of pussy willow ( Salix gracilistyla Miq.). The use of a twin screw extruder capable of continuous processing increased the solid loading to 50% in [EMIM]Ac and its co-solvent DMSO. The water-soluble fraction was increased by increasing the [EMIM]Ac amount in co-solvent and the extrusion temperature but decreasing the screw rotating speed and solid loading. The surface morphologies of the extruded product showed remarkable disruption of the cell wall structures and fibrillation with increasing amounts of [EMIM]Ac in co-solvent. With increasing [EMIM]Ac amount in co-solvent and extrusion temperature and with decreasing screw rotating speed and solid loading, the crystalline structure of cellulose was more disrupted, resulting in the increase in saccharification yield. The highest yield of glucose and xylose was 99.0% and 99.5%, respectively, from the pretreated products using only [EMIM]Ac under solid loading of 15% at 160 °C extrusion temperature and 5 rpm screw rotating speed. The initial saccharification rate was faster in the pretreated product precipitated in alcohol and acetone than in water.

中文翻译：

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提高离子液体预处理效果的挤压工艺以改善木质纤维素生物质的酶促水解

在共溶剂二甲基亚砜 (DMSO) 中使用 1-乙基-3-甲基咪唑鎓醋酸盐 ([EMIM]Ac) 进行挤出工艺，以提高对褪色柳 (Salix gracilistyla Miq.) 酶促糖化的预处理效果。使用能够连续加工的双螺杆挤出机将 [EMIM]Ac 及其共溶剂 DMSO 中的固体负载增加到 50%。通过增加共溶剂中 [EMIM]Ac 的量和挤出温度，但降低螺杆转速和固体负载，水溶性分数增加。随着共溶剂中 [EMIM]Ac 含量的增加，挤出产品的表面形态显示出细胞壁结构的显着破坏和原纤维化。随着共溶剂中[EMIM]Ac用量的增加和挤出温度的增加，以及螺杆转速和固含量的降低，纤维素的晶体结构被更多地破坏，导致糖化产率增加。葡萄糖和木糖的最高产率分别为 99.0% 和 99.5%，在 160 °C 挤出温度和 5 rpm 螺杆转速下，仅使用 [EMIM] Ac 在固体负载为 15% 的情况下预处理产品。在乙醇和丙酮中沉淀的预处理产物的初始糖化速率比在水中更快。来自仅使用 [EMIM]Ac 在 160 °C 挤出温度和 5 rpm 螺杆转速下固体负载为 15% 的预处理产品。在乙醇和丙酮中沉淀的预处理产物的初始糖化速率比在水中更快。来自仅使用 [EMIM]Ac 在 160 °C 挤出温度和 5 rpm 螺杆转速下固体负载为 15% 的预处理产品。在乙醇和丙酮中沉淀的预处理产物的初始糖化速率比在水中更快。