Recent technical advances in genomic technology have led to the explosive growth of transcriptome-wide studies at the level of single cells. The review describes the first steps of the single cell proteomics that has originated soon after development of transcriptomics methods. The first studies on the shotgun proteomics of single cells that used liquid chromatography/mass spectrometry have been already published. In these works, the cells were separated by the methods used in transcriptomics studies (e.g., cell sorting) and analyzed by modified mass spectrometry with tandem mass tags. The new proteogenomics approach involving integration of single cell transcriptomics and proteomics data will provide better understanding of the mechanisms of cell interactions in normal development and disease.

中文翻译：

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单细胞蛋白质基因组学 — 近期前景

基因组技术的最新技术进步导致单细胞水平的转录组范围研究的爆炸性增长。该综述描述了转录组学方法发展后不久的单细胞蛋白质组学的第一步。使用液相色谱/质谱法的单细胞鸟枪蛋白质组学的第一项研究已经发表。在这些工作中，细胞通过转录组学研究中使用的方法（例如细胞分选）分离，并通过带有串联质量标签的改良质谱进行分析。新的蛋白质基因组学方法涉及整合单细胞转录组学和蛋白质组学数据，将提供对正常发育和疾病中细胞相互作用机制的更好理解。