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Sporadic cyclosporiasis in symptomatic Cuban patients: Confirmation of positive results from conventional diagnostic methods by molecular assay.
Diagnostic Microbiology and Infectious Disease ( IF 2.1 ) Pub Date : 2020-03-26 , DOI: 10.1016/j.diagmicrobio.2020.115048
Luis Enrique Jerez Puebla 1 , Fidel A Núñez Fernández 2 , Jorge Fraga Nodarse 1 , Iraís Atencio Millán 1 , Iredys Cruz Rodríguez 1 , Lisette Pérez Santos 3 , Yanet Pintos Saavedra 3 , Laura Rodríguez Moreno 1 , Lucy J Robertson 4
Affiliation  

In Cuba, there are few studies on cyclosporiasis. Here, we report results from 1247 stool samples from symptomatic patients that were examined by microscopy methods and positive cases confirmed by nested PCR targeting the 18S rRNA gene, followed by sequencing. Seven positive samples, all diagnosed during May-June, were confirmed by the molecular method, indicating an occurrence in this patient cohort of 0.56%.

中文翻译:

有症状的古巴患者中偶发的环孢子虫病:通过分子分析证实了常规诊断方法的阳性结果。

在古巴,关于孢子菌病的研究很少。在这里,我们报告了来自有症状患者的1247份粪便样品的结果,这些结果通过显微镜方法进行了检查,阳性病例通过靶向18S rRNA基因的巢式PCR证实,随后进行了测序。通过分子方法确认了全部在5月至6月期间被诊断出的七个阳性样本,表明该患者队列的发生率为0.56%。
更新日期:2020-04-20
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