We expanded our current Centers for Disease Control and Prevention triplexed real-time polymerase chain reaction scheme identifying 11 individual serotypes and 10 serogroups to a quadriplex format identifying 34 individual serotypes and 13 small serogroups, 4 antibiotic resistance determinants, pilus targets, and penicillin susceptibility. Newly developed assays are specific for serotypes/serogroups, are sensitive (10 copies/reaction), and further discriminate larger serogroups into individual serotypes or smaller serogroups.

中文翻译：

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扩展的顺序四重实时荧光聚合酶链反应（PCR），用于鉴定肺炎球菌血清型，青霉素敏感性和耐药标记。

我们将目前的疾病预防控制中心三重实时聚合酶链式反应方案（可识别11种个体血清型和10种血清群）扩展为四线体形式，可识别34种个体血清型和13种小血清群，4种抗生素耐药性决定因素，菌毛靶标和青霉素敏感性。新开发的测定法对血清型/血清型是特异性的，是敏感的（10拷贝/反应），并且进一步将较大的血清型区分为单独的血清型或较小的血清型。