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Expanded sequential quadriplex real-time polymerase chain reaction (PCR) for identifying pneumococcal serotypes, penicillin susceptibility, and resistance markers.
Diagnostic Microbiology and Infectious Disease ( IF 2.1 ) Pub Date : 2020-03-12 , DOI: 10.1016/j.diagmicrobio.2020.115037
Srinivasan Velusamy 1 , Theresa Tran 2 , Thitipong Mongkolrattanothai 3 , Hollis Walker 1 , Lesley McGee 1 , Bernard Beall 1
Affiliation  

We expanded our current Centers for Disease Control and Prevention triplexed real-time polymerase chain reaction scheme identifying 11 individual serotypes and 10 serogroups to a quadriplex format identifying 34 individual serotypes and 13 small serogroups, 4 antibiotic resistance determinants, pilus targets, and penicillin susceptibility. Newly developed assays are specific for serotypes/serogroups, are sensitive (10 copies/reaction), and further discriminate larger serogroups into individual serotypes or smaller serogroups.

中文翻译:

扩展的顺序四重实时荧光聚合酶链反应(PCR),用于鉴定肺炎球菌血清型,青霉素敏感性和耐药标记。

我们将目前的疾病预防控制中心三重实时聚合酶链式反应方案(可识别11种个体血清型和10种血清群)扩展为四线体形式,可识别34种个体血清型和13种小血清群,4种抗生素耐药性决定因素,菌毛靶标和青霉素敏感性。新开发的测定法对血清型/血清型是特异性的,是敏感的(10拷贝/反应),并且进一步将较大的血清型区分为单独的血清型或较小的血清型。
更新日期:2020-04-20
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