Viral respiratory infections remain a significant cause of morbidity and mortality in pediatric, elderly, and immunocompromised patients. The Panther Fusion respiratory panels consist of 3 separate multiplex assays that test for 1) influenza A, influenza B, and RSV; 2) parainfluenza virus types 1-4; or 3) adenovirus, human metapneumovirus, and rhinovirus. This study evaluated the performance of the Fusion assays for both upper and lower respiratory tract specimens in comparison to routine methods, including viral culture and targeted real-time polymerase chain reaction assays. Following discordant resolution, the Fusion assays demonstrated high overall correlation (98.6% [648/657]) with routine methods. In addition, prospective testing of respiratory specimens (n = 146) submitted for viral culture showed a ~10-fold increase in detection by the Fusion panels compared to viral culture (28.1% versus 2.7% positivity). The Fusion respiratory panels offer a flexible, more targeted approach to respiratory virus testing with a turnaround time comparable to other molecular assays.

中文翻译：

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Panther Fusion呼吸板与常规方法的比较，用于检测上下呼吸道标本中的病毒。

病毒性呼吸道感染仍然是小儿，老年人和免疫功能低下患者发病和死亡的重要原因。Panther Fusion呼吸系统检测面板由3种独​​立的多重分析组成，可测试1）甲型流感，乙型流感和RSV；2）1-4型副流感病毒；或3）腺病毒，人间质肺病毒和鼻病毒。与常规方法（包括病毒培养和靶向实时聚合酶链反应分析）相比，该研究评估了上，下呼吸道标本融合检测的性能。在不一致的解决方案之后，Fusion分析与常规方法显示出很高的总体相关性（98.6％[648/657]）。此外，提交给病毒培养的呼吸道标本（n = 146）的前瞻性测试显示，与病毒培养相比，Fusion面板的检测结果增加了约10倍（阳性率分别为28.1％和2.7％）。Fusion呼吸小组为呼吸道病毒测试提供了一种灵活，针对性更强的方法，其周转时间可与其他分子分析媲美。