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Karyotypic instability of endoprophase and mitotic cells of Amoeba sp. strain Cont from the "proteus-type" group (Amoebozoa, Euamoebida, Amoebidae).
European Journal of Protistology ( IF 1.9 ) Pub Date : 2020-03-10 , DOI: 10.1016/j.ejop.2020.125691
Sergei Yu Demin 1 , Mariia A Berdieva 1 , Yuliya I Podlipaeva 1 , Andrew V Goodkov 1
Affiliation  

We performed karyotyping of Amoeba sp. strain Cont. Based on the results of a cytological analysis, we concluded that the chromosome number of Amoeba sp. strain Cont in mitosis was unstable. In all cases they appeared to be hypergaploid (the basic chromosome number is 30), with monosomy of all chromosomes except four shortest ones. The presence of “extrachromosomes” in the nucleus could prolong until the beginning of the anaphase. It was only then that they were ejected from the nucleus and the euploidy (haploidy) was restored. The stage of endoprophase nucleus was revealed in the cell cycle of Amoeba sp. strain Cont. This stage has not yet been found in other amoebae from the “proteus-type” group that had been previously studied (A. proteus strain B and A. borokensis). The maximum number of endoreplication rounds in the strain Cont amoebae nuclear cycle was 4 or 5. The regular extrusion of chromosomes from the nucleus into the cytoplasm occurred in each of the endoreplication rounds. Comparative cytological analysis of A. proteus strain B, A. borokensis and Amoeba sp. strain Cont karyotypes indicated that strain Cont, though rather close to the former two amoebae, is actually a distinct species.



中文翻译:

变形虫变形虫内核和分裂细胞的核型不稳定性。“变形型”组(变形虫,Euamoebida,Amoebidae)中的Cont菌株。

我们进行了变形虫的核型分析。应变续 根据细胞学分析的结果,我们得出结论,变形虫的染色体数。有丝分裂中的Cont菌株不稳定。在所有情况下,它们似乎都是超gaploid(基本染色体数为30),除四个最短的染色体外,所有染色体均具有单体性。细胞核中“超染色体”的存在可能会延长到后期。直到那时,它们才从细胞核中弹出,并恢复整倍性(单倍性)。在变形虫的细胞周期中揭示了前期核的阶段。应变续 此阶段尚未在先前研究过的“变形型”组的其他变形虫中发现(A.变形杆菌B和A.borokensis)。菌株变形虫核周期中的最大内转轮数为4或5。在每个内转轮轮中都发生染色体从核到细胞质的规则挤压。变形杆菌A,B。borokensisAmoeba sp。的比较细胞学分析。菌株Cont的核型表明,菌株Cont虽然与前两个变形虫非常接近,但实际上是一个独特的物种。

更新日期:2020-03-10
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