Arctium lappa root extract containing L-arginine prevents TNF-α-induced early atherosclerosis in vitro and in vivo,Nutrition Research

当前位置： X-MOL 学术 › Nutr. Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Arctium lappa root extract containing L-arginine prevents TNF-α-induced early atherosclerosis in vitro and in vivo
Nutrition Research ( IF 3.4 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.nutres.2020.03.003
Jangho Lee ₁ , Su Jeong Ha ₂ , Joon Park ₃ , Young Ho Kim ₄ , Nam Hyouck Lee ₄ , Young Eon Kim ₄ , Young-Shick Hong ₅ , Kyung-Mo Song ₄

Affiliation

Atherosclerosis is a chronic inflammatory disease affecting the aorta and is a major cause of cardiovascular disease. Arctium lappa root is a plant widely used in traditional Chinese medicine (TCM), and Arctium lappa root extract (ALE) has been reported to exhibit anti-inflammatory capacity and to ameliorate endothelial dysfunction. Thus, we hypothesized that ALE would inhibit the early atherosclerotic stage. In this study, we evaluated the inhibitory effect of ALE on early arteriosclerosis and its mechanisms of action. ALE suppressed TNF-α-induced monocyte adhesion to the vascular endothelium by suppressing NF-κB signaling in HUVECs. In an acute mouse model of atherosclerosis, ALE suppressed TNF-α-induced monocyte infiltration of the vascular endothelium and the expression of genes encoding inflammatory cytokines including IL-1β, IL-6, TNF-α, and MCP-1 in the mouse aorta. Moreover, inulin-type fructan and amino acids, especially L-aspartate and L-arginine (60.27 and 42.17 mg/g, respectively) were detected by NMR, MALDI-TOF MS, and HPLC analysis as the main components of ALE. Notably, L-arginine suppressed TNF-α-induced monocyte adhesion to HUVECs. Therefore, these results suggest that ALE may be a functional food for the suppression or prevention of early stages of atherosclerosis.

中文翻译：

含有 L-精氨酸的牛蒡根提取物在体外和体内预防 TNF-α 诱导的早期动脉粥样硬化

动脉粥样硬化是一种影响主动脉的慢性炎症性疾病，是心血管疾病的主要原因。牛蒡根是一种广泛用于中药 (TCM) 的植物，据报道牛蒡根提取物 (ALE) 具有抗炎能力和改善内皮功能障碍。因此，我们假设 ALE 会抑制早期动脉粥样硬化阶段。在本研究中，我们评估了 ALE 对早期动脉硬化的抑制作用及其作用机制。ALE 通过抑制 HUVEC 中的 NF-κB 信号传导来抑制 TNF-α 诱导的单核细胞对血管内皮的粘附。在动脉粥样硬化的急性小鼠模型中，ALE 抑制 TNF-α 诱导的血管内皮单核细胞浸润和编码炎性细胞因子（包括 IL-1β、IL-6、TNF-α、和小鼠主动脉中的 MCP-1。此外，通过NMR、MALDI-TOF MS和HPLC分析，菊粉型果聚糖和氨基酸，尤其是L-天冬氨酸和L-精氨酸（分别为60.27和42.17 mg/g）作为ALE的主要成分被检测到。值得注意的是，L-精氨酸抑制了 TNF-α 诱导的单核细胞对 HUVEC 的粘附。因此，这些结果表明ALE可能是抑制或预防早期动脉粥样硬化的功能性食品。

更新日期：2020-05-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11