Hypsizygus marmoreus is an important commercial edible fungus, but the lack of basic studies on this fungus has hindered further development of its commercial value. In this study, we found that the treatment of damaged vegetative mycelia with 1 mM l-ascorbic acid (ASA) significantly increased the antioxidant enzyme activities (GPX, GR, CAT and SOD) and antioxidant contents (GSH and ASA) and reduced the ROS levels (H2O2 and O2-) in mechanically damaged mycelia. Additionally, this treatment increased mycelial biomass. At the reproductive stage, our results demonstrated that the treatment of damaged H. marmoreus mycelia with 2.24 mM ASA significantly increased the antioxidant enzyme activities (GPX, GR, GST, TRXR and CAT), endogenous ASA contents and GSH/GSSG ratios in different developmental stages and significantly decreased the MDA and H2O2 contents. Furthermore, this study showed that the expression levels of the antioxidant enzyme genes were consistent with the enzyme activities. Damaged mycelia treated with ASA regenerated 2-3 d earlier than the control group and showed significantly enhanced fruiting body production. These results suggested that exogenous ASA regulated mycelia intracellular ASA content to increase mycelial antioxidant abilities, induce the regeneration of damaged mycelia and regulate the development of fruiting bodies in H. marmoreus.

中文翻译：

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外源性L-抗坏血酸调节抗氧化系统，促进受损菌丝体的再生并诱导Hypsizygus marmoreus子实体的发育

Hypsizygus marmoreus 是一种重要的商业食用菌，但对该菌缺乏基础研究，阻碍了其商业价值的进一步开发。在本研究中，我们发现用 1 mM l-抗坏血酸 (ASA) 处理受损的营养菌丝体可显着增加抗氧化酶活性（GPX、GR、CAT 和 SOD）和抗氧化剂含量（GSH 和 ASA）并降低 ROS机械损伤菌丝体中的水平（H2O2 和 O2-）。此外，这种处理增加了菌丝体生物量。在繁殖阶段，我们的结果表明，用 2.24 mM ASA 处理受损的海棠菌丝体显着增加了抗氧化酶活性（GPX、GR、GST、TRXR 和 CAT），内源性 ASA 含量和 GSH/GSSG 比值在不同发育阶段显着降低 MDA 和 H2O2 含量。此外，该研究表明抗氧化酶基因的表达水平与酶活性一致。用ASA处理的受损菌丝体比对照组早2-3天再生并且显示出显着增强的子实体产生。这些结果表明外源ASA调节菌丝体细胞内ASA含量以增加菌丝体抗氧化能力，诱导受损菌丝体再生并调节H. marmoreus子实体的发育。用ASA处理的受损菌丝体比对照组早2-3天再生并且显示出显着增强的子实体产生。这些结果表明外源ASA调节菌丝体细胞内ASA含量以增加菌丝体抗氧化能力，诱导受损菌丝体再生并调节H. marmoreus子实体的发育。用ASA处理的受损菌丝体比对照组早2-3天再生并且显示出显着增强的子实体产生。这些结果表明外源ASA调节菌丝体细胞内ASA含量以增加菌丝体抗氧化能力，诱导受损菌丝体再生并调节H. marmoreus子实体的发育。