Small heat shock proteins (sHsps) are molecular chaperones of low molecular weight involved in an early association with misfolded proteins. In response to heat shock, B. emersonii induces the synthesis of a number of proteins. As sHsps are still poorly studied in B. emersonii and in fungi overall, the aim of this work was to carry out a in-depth characterization of sHsps during B. emersonni life cycle, as well as in response to thermal stress. We verified a strong induction of the hsp17 gene in cells exposed to heat shock both in germination and sporulation stages, and that Hsp17 protein levels show the same pattern of variation of its mRNA. Unlike hsp17 and hsp30, hsp16 gene is not significantly induced during heat shock, in germination or sporulation cells. However, at normal temperatures, the hsp16 gene presents high mRNA levels in sporulation cells, whereas the hsp30 gene presents high mRNA levels in germination cells. Interestingly, heat shock mRNA levels for hsp17 and hsp30 genes are 10 times higher in germination cells than in sporulation cells. Thus, our data show that the expression of these sHsp genes is quite distinct, both under normal temperature as during heat shock.

中文翻译：

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小热休克蛋白基因在艾默森芽孢杆菌的应激和非应激条件下受到发育调控

小热休克蛋白 (sHsps) 是低分子量的分子伴侣，参与与错误折叠蛋白的早期关联。响应热休克，B. emersonii 诱导多种蛋白质的合成。由于 sHsps 在 B. emersonii 和整个真菌中的研究仍然很少，这项工作的目的是在 B. emersonni 生命周期以及对热应力的响应中对 sHsps 进行深入表征。我们证实了在萌发和孢子形成阶段暴露于热休克的细胞中 hsp17 基因的强烈诱导，并且 Hsp17 蛋白水平显示其 mRNA 的相同变异模式。与 hsp17 和 hsp30 不同，在热休克、发芽或孢子形成细胞中，hsp16 基因不会被显着诱导。然而，在常温下，hsp16 基因在孢子形成细胞中呈现高 mRNA 水平，而 hsp30 基因在萌发细胞中呈现高 mRNA 水平。有趣的是，发芽细胞中 hsp17 和 hsp30 基因的热休克 mRNA 水平是孢子形成细胞中的 10 倍。因此，我们的数据显示这些 sHsp 基因的表达非常不同，无论是在正常温度下还是在热休克期间。