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The Vta1 transcriptional regulator is required for microsclerotia melanization in Verticillium dahliae
Fungal Biology ( IF 2.9 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.funbio.2020.01.007
Rebekka Harting 1 , Annalena Höfer 1 , Van-Tuan Tran 1 , Lisa-Maria Weinhold 1 , Sina Barghahn 1 , Rabea Schlüter 2 , Gerhard H Braus 1
Affiliation  

Many fungi are able to produce resting structures, which ensure survival and protect them against various stresses in their habitat such as exposure to UV light, temperature variations, drought as well as changing pH and nutrient conditions. Verticillium dahliae is a plant pathogenic fungus that forms melanized resting structures, called microsclerotia, for survival of time periods without a host. These highly stress resistant microsclerotia persist in the soil for many years and are therefore problematic for an effective treatment of the fungus. The Verticillium transcription activator of adhesion 1 (Vta1) was initially identified as one of several transcriptional regulators that rescue adhesion in non-adhesive Saccharomyces cerevisiae cells. Vta2 and Vta3 are required for early steps in plant infection and colonization and additionally control microsclerotia formation. Here, we show that Vta1 function is different, because it is dispensable for root colonization and infection. Vta1 is produced in the fungal cell during microsclerotia development. Analysis of the deletion mutant revealed that the absence of Vta1 allows microsclerotia production, but they are colorless and no more melanized. Vta1 is required for melanin production and activates transcription of melanin biosynthesis genes including the polyketide synthase encoding PKS1 and the laccase LAC1. The primary function of Vta1 in melanin production is important for survival of microsclerotia as resting structures of V. dahliae.

中文翻译:

Vta1 转录调节因子是大丽轮枝菌微菌核黑化所必需的

许多真菌能够产生静止结构,以确保生存并保护它们免受栖息地中的各种压力,例如暴露于紫外线、温度变化、干旱以及不断变化的 pH 值和营养条件。Verticillium dahliae 是一种植物病原真菌,它形成黑色化的静止结构,称为微菌核,可在没有宿主的情况下存活一段时间。这些高度抗逆的微菌核在土壤中持续存在多年,因此对于真菌的有效处理存在问题。Verticillium 转录激活剂粘附 1 (Vta1) 最初被确定为几种转录调节因子之一,可在非粘附性酿酒酵母细胞中拯救粘附。Vta2 和 Vta3 是植物感染和定植的早期步骤所必需的,此外还控制微菌核的形成。在这里,我们表明 Vta1 功能是不同的,因为它对于根的定植和感染是可有可无的。Vta1 在微菌核发育过程中在真菌细胞中产生。对缺失突变体的分析表明,Vta1 的缺失允许产生微菌核,但它们是无色的,不再被黑化。Vta1 是黑色素生成所必需的,它激活黑色素生物合成基因的转录,包括编码 PKS1 和漆酶 LAC1 的聚酮合酶。Vta1 在黑色素生成中的主要功能对于作为大丽花的静息结构的微菌核的存活很重要。因为它对于根部定植和感染是可有可无的。Vta1 在微菌核发育过程中在真菌细胞中产生。对缺失突变体的分析表明,Vta1 的缺失允许产生微菌核,但它们是无色的,不再被黑化。Vta1 是黑色素生成所必需的,它激活黑色素生物合成基因的转录,包括编码 PKS1 和漆酶 LAC1 的聚酮合酶。Vta1 在黑色素生成中的主要功能对于作为大丽花的静息结构的微菌核的存活很重要。因为它对于根部定植和感染是可有可无的。Vta1 在微菌核发育过程中在真菌细胞中产生。对缺失突变体的分析表明,Vta1 的缺失允许产生微菌核,但它们是无色的,不再被黑化。Vta1 是黑色素生成所必需的,它激活黑色素生物合成基因的转录,包括编码 PKS1 和漆酶 LAC1 的聚酮合酶。Vta1 在黑色素生成中的主要功能对于作为大丽花的静息结构的微菌核的存活很重要。Vta1 是黑色素生成所必需的,它激活黑色素生物合成基因的转录,包括编码 PKS1 和漆酶 LAC1 的聚酮合酶。Vta1 在黑色素生成中的主要功能对于作为大丽花的静息结构的微菌核的存活很重要。Vta1 是黑色素生成所必需的,它激活黑色素生物合成基因的转录,包括编码 PKS1 和漆酶 LAC1 的聚酮合酶。Vta1 在黑色素生成中的主要功能对于作为大丽花的静息结构的微菌核的存活很重要。
更新日期:2020-05-01
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