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On the role of the global regulator RlcA in red-light sensing in Aspergillus nidulans
Fungal Biology ( IF 2.9 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.funbio.2019.12.009
Zhenzhong Yu 1 , Jennifer Hübner 2 , Satur Herrero 2 , Victor Gourain 3 , Reinhard Fischer 2
Affiliation  

A large proportion of fungal genomes are under the control of light. Most fungi employ complex light sensing systems, consisting of red-, blue-, and in some cases green-light photoreceptors. Here we studied the light response in Aspergillus nidulans. In a genetic screen, followed by whole-genome sequencing we identified a global regulator, which appears to be involved in chromatin structure modification. We therefore named the protein RlcA (regulator of light sensing and chromatin remodeling). The protein comprises a nuclear localization signal, a PHD (plant homeodomain) finger, a TFSII (found in the central region of the transcription elongation factor S-II), and a SPOC domain (Spen paralog and ortholog C-terminal domain). In the mutant, where light-controlled genes were constitutively active, the SPOC domain is missing. RlcA localized to the nucleus and interacted with the phytochrome FphA. The PHD-finger domain probably binds to trimethylated lysine 4 of histone H3, whereas the TFSII domain binds RNA polymerase II. The SPOC domain could mediate interaction with a global repressor protein. In the mutant, repressor recruitment would be hindered, whereas in the wild type repressor release would be induced after light stimulation. Our results add another layer of complexity to light sensing in filamentous fungi.

中文翻译:

全局调节因子 RlcA 在构巢曲霉红光感知中的作用

大部分真菌基因组都在光的控制下。大多数真菌采用复杂的光感应系统,由红光、蓝光和某些情况下的绿光光感受器组成。在这里,我们研究了构巢曲霉中的光响应。在遗传筛选和全基因组测序中,我们确定了一个全局调节器,它似乎与染色质结构修饰有关。因此,我们将蛋白质命名为 RlcA(光感应和染色质重塑调节剂)。该蛋白质包含核定位信号、PHD(植物同源域)指、TFSII(发现于转录延伸因子 S-II 的中心区域)和 SPOC 域(Spen 旁系同源和直系同源 C 端域)。在光控基因组成性活跃的突变体中,SPOC 结构域缺失。RlcA 定位于细胞核并与光敏色素 FphA 相互作用。PHD-finger 结构域可能与组蛋白 H3 的三甲基化赖氨酸 4 结合,而 TFSII 结构域与 RNA 聚合酶 II 结合。SPOC 域可以介导与全局阻遏蛋白的相互作用。在突变体中,阻遏物的募集会受到阻碍,而在野生型中,光刺激后会诱导阻遏物的释放。我们的结果为丝状真菌中的光感应增加了另一层复杂性。而在野生型中,光刺激后会诱导阻遏物释放。我们的结果为丝状真菌中的光感应增加了另一层复杂性。而在野生型中,光刺激后会诱导阻遏物释放。我们的结果为丝状真菌中的光感应增加了另一层复杂性。
更新日期:2020-05-01
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