当前位置：
X-MOL 学术
›
Tuberculosis
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Phylogenomic assessment of drug-resistant Mycobacterium tuberculosis strains from Beira, Mozambique
Tuberculosis ( IF 2.8 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.tube.2020.101905 Evangelina Inacio Namburete , Anzaan Dippenaar , Emilyn Costa Conceição , Cinara Feliciano , Margarida Maria Passeri do Nascimento , Kamila Chagas Peronni , Wilson Araújo Silva , Josefo João Ferro , Lee H. Harrison , Robin Mark Warren , Valdes Roberto Bollela
Tuberculosis ( IF 2.8 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.tube.2020.101905 Evangelina Inacio Namburete , Anzaan Dippenaar , Emilyn Costa Conceição , Cinara Feliciano , Margarida Maria Passeri do Nascimento , Kamila Chagas Peronni , Wilson Araújo Silva , Josefo João Ferro , Lee H. Harrison , Robin Mark Warren , Valdes Roberto Bollela
BACKGROUND
Mozambique is a high-burden tuberculosis (TB) country where TB/HIV co-infection and drug resistant TB (DR-TB) incidence is increasing. Whole genome sequencing (WGS) comprehensively describes the molecular epidemiology of TB, allows prediction of DR-TB phenotypes, lineages strains identification and better understanding of transmission chains. OBJECTIVE
To describe genetic diversity of DR-TB Mycobacterium tuberculosis isolated in Beira, Mozambique. METHODS
Descriptive cross-sectional study with 35 M. tuberculosis isolates, resistant to at least one first-line drug on molecular drug-susceptibility tests (DST). Variant identification, DR prediction and phylogenetic analysis provided by WGS, drug-susceptibility pattern compared to line-probe assay (LPA): Genotype MTBDRTMplus and MTBDRTMsl. FINDINGS
Lineage 4 (L4) was the most prevalent: 25 (71.4%) isolates; 5 (14.3%) L1 and 5 (14.3%) L2. WGS showed 33/35 (94.3%) isolates resistant to at least one drug, two pan-susceptible isolates that were previously diagnosed as DR-TB with genotype MTBDRplus. Concordance between WGS and LPA: 88.6% for isoniazid (INH), 85.7% to rifampicin (RPM), 91.4% for quinolones and 100% to second line injectable drugs. There were three possible TB transmission chains, 10 strains showing recent transmission. CONCLUSION
WGS provided reliable information about the most frequent lineages related to DR-TB in Beira, Mozambique: L4.3 (LAM), L2 (Beijing) and L1 (EAI) and possible recent transmission chain.
更新日期：2020-03-01
京公网安备 11010802027423号