Macrophages are essential components of the immune system. Macrophages can be derived from the bone marrow of mice with either recombinant M-CSF or L929 supernatant. Recent literature considers recombinant M-CSF- and L929-derived macrophages as equals, even though L929-derived macrophages are exposed to other substances secreted in the L929 supernatant, and not only M-CSF. Thus, we decided to perform a comparative analysis of both inflammatory and metabolic profiles of macrophages differentiated under the aforementioned conditions, which is relevant for standardization and interpretation of in vitro studies. We observed that, when treated with LPS, L929macs secrete lower levels of proinflammatory cytokines (TNF-α, IL-6, IL12) and present higher glycolysis and oxygen consumption when compared with M-CSFmacs. L929macs also have increased mitochondrial mass, with higher percentage of dysfunctional mitochondria. This sort of information can help direct further studies towards a more specific approach for macrophage generation.

巨噬细胞是免疫系统的重要组成部分。巨噬细胞可以用重组 M-CSF 或 L929 上清液从小鼠的骨髓中提取。最近的文献认为重组 M-CSF 和 L929 衍生的巨噬细胞是平等的，即使 L929 衍生的巨噬细胞暴露于 L929 上清液中分泌的其他物质，而不仅仅是 M-CSF。因此，我们决定对在上述条件下分化的巨噬细胞的炎症和代谢特征进行比较分析，这与体外研究的标准化和解释有关。我们观察到，与 M-CSFmacs 相比，当用 LPS 处理时，L929macs 分泌较低水平的促炎细胞因子（TNF-α、IL-6、IL12）并呈现更高的糖酵解和耗氧量。L929macs 的线粒体质量也增加，线粒体功能障碍的百分比更高。这类信息可以帮助指导进一步的研究，寻找更具体的巨噬细胞生成方法。