The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueous phase were used. The prepared microemulsions were evaluated for clarity, pH, viscosity and FA content. Moreover, stability, sterility, antibacterial activity, in vitro release of the formulations were also evaluated. The results showed that the FA loaded microemulsion and microemulsion based gel formation and characteristics were related to many parameters of the components. The performed optimized microemulsion-based gel showed good stability over a period of 3 months. The antibacterial activity of microemulsion-based gel was found to be comparable with marketed cream. RAW 264.7 macrophages were used to determine cell viability (MTT assay) and nitric oxide production. MBG and FA-MBG significantly inhibit the production of the inflammatory mediator NO in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. The wound healing property was evaluated by histopathological examination and by measuring the wound contraction. The % of wound area in rats treated with FA (2%) loaded microemulsion based gel ranged from 69.30% to 41.39% in the period from 3 to 10 days. In conclusion, FA loaded microemulsion based gel could be offered as encouraging strategy as dermal systems for the burn wound treatment.

本研究的目的是研究基于微乳液的夫西地酸（FA）皮肤应用作为烧伤创面治疗的凝胶。在制备微乳液期间，使用油酸乙酯作为油相，吐温80作为表面活性剂，乙醇作为助表面活性剂，水作为水相。评价所制备的微乳液的透明度，pH，粘度和FA含量。此外，体外的稳定性，无菌性，抗菌活性还评估了制剂的释放。结果表明，负载FA的微乳液和基于微乳液的凝胶的形成和特性与组分的许多参数有关。进行的优化的基于微乳液的凝胶在3个月的时间内显示出良好的稳定性。发现基于微乳液的凝胶的抗菌活性与市售乳膏相当。RAW 264.7巨噬细胞用于确定细胞活力（MTT分析）和一氧化氮的产生。MBG和FA-MBG以浓度依赖的方式显着抑制LPS刺激的RAW 264.7细胞中炎性介质NO的产生。通过组织病理学检查和通过测量伤口收缩来评估伤口愈合特性。在3至10天的时间内，用FA（2％）负载的基于微乳的凝胶处理的大鼠的伤口面积％在69.30％至41.39％的范围内。总之，作为皮肤烧伤创面治疗系统的FA载微乳胶可以作为鼓励策略。