aurJ3M(GenBank:EU697915.1), a 579 bp gene, whose deduced product (192 amino acid) was found to have amino acid sequence homology with some bacterial regulatory proteins. Computer-assisted analysis showed that AurJ3M is PAS-LuxR family regulatory protein, it combines a PAS domain with a helix-turn-helix (HTH) motif of the LuxR type. Gene deletion of aurJ3M from the S.aureofuscus SYAU0709 through gene replacement with the apramycin resistance gene aac (3) IV and the DNA fragment of the replication initiation site sequence OriT resulted in complete loss of aureofuscin production, indicating that AurJ3M is a positive regulator during the aureofuscin biosynthesis. Gene expression analyses in S.aureofuscus SYAU0709 and S.aureofuscus ΔaurJ3M by reverse transcriptase PCR (RT-PCR) indicated there no transcripts for the genes B、C、G、F、S0、S1、D in S.aureofuscus ΔaurJ3M, maintains some transcription of these genes although the levels is low. Transcription was also significantly reduced for gene A, and no difference in the transcription pattern was observed for the genes R、E and H, a similar transcription pattern was also observed for AurJ3M, A and B showed a different transcription pattern, while transcription of gene A in S.aureofuscus ΔaurJ3M when compared to the parental strain, no transcripts could be detected for gene B in the mutant; This result indicates that the deletion of aurJ3M gene doesn't have a polar effect on the transcription of genes located downstream from aurJ3M. In the ΔaurJ3M knockout mutant, except for the transcription of E、H、M and R, the transcription of other genes in the cluster was down-regulated, HPLC shows there no secondary metabolites was produced,aurJ3M has a pathway-specific regulation effect on the biosynthesis of aureofuscin. Aureofuscin had broad application prospects in food preservation, and enriched the resource pool of anti-fungal antibiotics of tetraphenyl macrolide in China.

中文翻译：

---

PAS-LuxR 系列调节器 AurJ3M 对金黄素生产的调节

aurJ3M(GenBank:EU697915.1)，一个579 bp的基因，其推导产物(192个氨基酸)被发现与一些细菌调节蛋白具有氨基酸序列同源性。计算机辅助分析表明，AurJ3M 是 PAS-LuxR 家族调节蛋白，它结合了 PAS 结构域和 LuxR 类型的螺旋-转角-螺旋 (HTH) 基序。通过用安普霉素抗性基因 aac (3) IV 和复制起始位点序列 OriT 的 DNA 片段进行基因置换，金黄色葡萄球菌 SYAU0709 的 aurJ3M 基因缺失导致金黄色素产生完全丧失，表明 AurJ3M 在金黄素生物合成。通过逆转录酶聚合酶链反应（RT-PCR）在金黄色葡萄球菌SYAU0709和金黄色葡萄球菌ΔaurJ3M中的基因表达分析表明，在金黄色葡萄球菌中没有基因B、C、G、F、S0、S1、D的转录物。aureofuscus ΔaurJ3M 维持这些基因的一些转录，尽管水平很低。基因A的转录也显着减少，基因R、E和H的转录模式没有差异，AurJ3M也观察到相似的转录模式，A和B表现出不同的转录模式，而基因的转录S.aureofuscus ΔaurJ3M 中的 A 与亲本菌株相比，在突变体中没有检测到基因 B 的转录物；该结果表明aurJ3M基因的缺失对位于aurJ3M下游的基因的转录没有极性影响。在 ΔaurJ3M 敲除突变体中，除了 E、H、M 和 R 的转录外，簇内其他基因的转录下调，HPLC 显示没有次级代谢产物产生，aurJ3M 对金黄素的生物合成具有通路特异性调控作用。金黄素在食品保鲜方面具有广阔的应用前景，丰富了我国四苯基大环内酯类抗真菌抗生素的资源库。