Abstract This paper presents a control architecture suitable for autotrophic cultivations in a stirred tank reactor feeding a gas mixture of H2/CO2/O2. No metabolic information about the cultivated strain is required for the gas phase controller, which can also be used as part of the overall control for the entire process. A gain-scheduled MIMO-PI controller connected to an adaptive feedforward disturbance rejection is proposed. The latter exploits the estimated gas transfer rates of a Kalman filter solely based on a description of the non-biotic processes occurring. Thus, without further knowledge of the autotrophic strain, experiments can be run to generate data and to develop a process model including strain-specific biological details. The wild-type strain H16 of Ralstonia eutropha and two mutants, HF805 and H798, are used to demonstrate how this can be done.

中文翻译：

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用于富养产碱杆菌不同突变体自养培养的高级非线性气相控制

摘要 本文提出了一种适合在搅拌釜反应器中自养培养的控制架构，该反应器供给 H2/CO2/O2 的气体混合物。气相控制器不需要有关培养菌株的代谢信息，也可以用作整个过程整体控制的一部分。提出了一种连接到自适应前馈干扰抑制的增益调度 MIMO-PI 控制器。后者仅基于对发生的非生物过程的描述来利用卡尔曼滤波器的估计气体传输率。因此，无需进一步了解自养菌株，就可以运行实验以生成数据并开发包括菌株特定生物学细节的过程模型。Ralstonia eutropha 的野生型菌株 H16 和两个突变体，HF805 和 H798，