The generation of wild-type, mutant, and reassortant influenza viruses from viral cDNAs (reverse genetics) is now a basic molecular virology technique in many influenza virus laboratories. Here, I describe the original RNA polymerase I reverse genetics system and the modifications that have been developed in past years. Together, these technologies have made possible many advances in basic and applied influenza virology that would not have been otherwise attainable, including the revival and study of extinct influenza viruses, the rapid characterization of emerging influenza viruses, the generation of conventional influenza vaccines, and the development of novel influenza vaccines.

中文翻译：

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流行性感冒反向遗传学的历史研究

现在，从病毒cDNA（反向遗传学）中产生野生型，突变型和重配型流感病毒是许多流感病毒实验室中的基本分子病毒学技术。在这里，我描述了原始的RNA聚合酶I反向遗传学系统以及近几年来开发的修饰。总之，这些技术使基础和应用流感病毒学取得了许多原本无法实现的进步，包括复兴和研究已灭绝的流感病毒，快速鉴定新兴的流感病毒，生产常规流感疫苗以及开发新型流感疫苗。