Aesthetic wound healing is often experienced by patients after electrochemotherapy. We hypothesized that pulsed electric fields applied during electrochemotherapy (ECT) or gene electrotransfer (GET) protocols could stimulate proliferation and migration of human cutaneous cells, as described in protocols for electrostimulation of wound healing. We used videomicroscopy to monitor and quantify in real time primary human dermal fibroblast behavior when exposed in vitro to ECT and GET electric parameters, in terms of survival, proliferation and migration in a calibrated scratch wound assay. Distinct electric field intensities were applied to allow gradient in cell electropermeabilization while maintaining reversible permeabilization conditions, in order to mimic in vivo heterogeneous electric field distribution of complex tissues. Neither galvanotaxis nor statistical modification of fibroblast migration were observed in a calibrated scratch wound assay after application of ECT and GET parameters. The only effect on proliferation was observed under the strongest GET conditions, which drastically reduced the number of fibroblasts through induction of mitochondrial stress and apoptosis. Finally, we found that 24 h-conditioned cell culture medium by electrically stressed fibroblasts tended to increase the migration properties of cells that were not exposed to electric field. RT-qPCR array indicated that several growth factor transcripts were strongly modified after electroporation.

中文翻译：

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电穿孔不会直接影响人类皮肤成纤维细胞的增殖和迁移，但会通过分泌组间接影响。

化疗后，患者通常会经历美学伤口的愈合。我们假设在电化学疗法（ECT）或基因电转移（GET）方案中施加脉冲电场可以刺激人皮肤细胞的增殖和迁移，如伤口愈合电刺激方案中所述。我们使用视频显微镜实时监测和量化体外体外受ECT和GET电参数影响的原代人皮肤成纤维细胞的行为，包括在校准的刮伤试验中的存活，增殖和迁移情况。施加不同的电场强度以允许细胞电透化中的梯度，同时保持可逆的透化条件，以模拟复杂组织的体内异质电场分布。应用ECT和GET参数后，在校准的刮擦伤口试验中未观察到galvanotaxis或成纤维细胞迁移的统计变化。在最强的GET条件下观察到对增殖的唯一影响，该条件通过诱导线粒体应激和凋亡而大大减少了成纤维细胞的数量。最后，我们发现由电应激的成纤维细胞在24 h条件下培养的细胞培养基倾向于增加未暴露于电场的细胞的迁移特性。RT-qPCR芯片显示电穿孔后，一些生长因子转录物被强烈修饰。在最强的GET条件下观察到对增殖的唯一影响，该条件通过诱导线粒体应激和凋亡而大大减少了成纤维细胞的数量。最后，我们发现由电应激的成纤维细胞在24 h条件下培养的细胞培养基倾向于增加未暴露于电场的细胞的迁移特性。RT-qPCR芯片显示电穿孔后，一些生长因子转录物被强烈修饰。在最强的GET条件下观察到对增殖的唯一影响，该条件通过诱导线粒体应激和凋亡而大大减少了成纤维细胞的数量。最后，我们发现由电应激的成纤维细胞在24 h条件下培养的细胞培养基倾向于增加未暴露于电场的细胞的迁移特性。RT-qPCR芯片显示电穿孔后，一些生长因子转录物被强烈修饰。