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Recovery of antioxidant and antimicrobial peptides through the reutilization of Nile perch wastewater by biodegradation using two Bacillus species.
Chemosphere ( IF 8.1 ) Pub Date : 2020-04-07 , DOI: 10.1016/j.chemosphere.2020.126728 Changoma Fransis Mhina 1 , Hyun Yi Jung 2 , Joong Kyun Kim 3
Chemosphere ( IF 8.1 ) Pub Date : 2020-04-07 , DOI: 10.1016/j.chemosphere.2020.126728 Changoma Fransis Mhina 1 , Hyun Yi Jung 2 , Joong Kyun Kim 3
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Nile perch wastewater was biodegraded using two Bacillus species to recover bioactive substances to enhance its reutilization value. The two Bacillus species successfully produced low-molecular-weight substances with a 47.8% degree of hydrolysis. The antioxidant activities of the Nile perch wastewater increased as the biodegradation proceeded, and the culture supernatant exhibited the highest DPPH (80.1%), ABTS (93.1%) and Fe2+ chelating (88.5%) antioxidant activities at 60 h. The antioxidant potential of the biodegraded Nile perch wastewater was found to be higher than those of other fish hydrolysates. Moreover, the biodegraded Nile perch wastewater exhibited effective antimicrobial activity against Vibrio vulnificus, exhibiting a minimal inhibitory concentration of 585 μg mL-1. Two-dimensional thin layer chromatography analysis revealed the specific amino acids responsible for the antioxidant activity, and molecular-weight cut-off ultrafiltration revealed that the <2-kDa fraction exhibited the highest antioxidant activity with the lowest IC50 values (0.43 and 0.22 mg mL-1 for DPPH and ABTS antioxidant activities, respectively). This is the first report of the reutilization of Nile perch wastewater as a natural antioxidant and antimicrobial ingredient for nutraceuticals.
中文翻译：
通过使用两种芽孢杆菌属物种进行生物降解来重新利用尼罗河鲈鱼废水来回收抗氧化剂和抗菌肽。
使用两种芽孢杆菌物种对尼罗河鲈鱼废水进行了生物降解，以回收生物活性物质，以提高其再利用价值。这两个芽孢杆菌属物种成功生产了具有47.8％水解度的低分子量物质。尼罗河鲈鱼废水的抗氧化活性随着生物降解的进行而增加，并且在60 h时，培养上清液表现出最高的DPPH（80.1％），ABTS（93.1％）和Fe2 +螯合（88.5％）的抗氧化活性。发现生物降解的尼罗河鲈鱼废水的抗氧化潜力高于其他鱼类水解产物。此外，经过生物降解的尼罗河鲈鱼废水对创伤弧菌表现出有效的抗菌活性，最低抑制浓度为585μgmL-1。二维薄层色谱分析揭示了负责抗氧化活性的特定氨基酸，分子量截留超滤表明，<2-kDa馏分具有最高的抗氧化活性，而IC50值最低（0.43和0.22 mg mL -1分别表示DPPH和ABTS的抗氧化活性）。这是尼罗河鲈鱼废水作为天然抗氧化剂和营养成分被再利用的第一份报告。
更新日期：2020-04-08
中文翻译：
通过使用两种芽孢杆菌属物种进行生物降解来重新利用尼罗河鲈鱼废水来回收抗氧化剂和抗菌肽。
使用两种芽孢杆菌物种对尼罗河鲈鱼废水进行了生物降解，以回收生物活性物质，以提高其再利用价值。这两个芽孢杆菌属物种成功生产了具有47.8％水解度的低分子量物质。尼罗河鲈鱼废水的抗氧化活性随着生物降解的进行而增加，并且在60 h时，培养上清液表现出最高的DPPH（80.1％），ABTS（93.1％）和Fe2 +螯合（88.5％）的抗氧化活性。发现生物降解的尼罗河鲈鱼废水的抗氧化潜力高于其他鱼类水解产物。此外，经过生物降解的尼罗河鲈鱼废水对创伤弧菌表现出有效的抗菌活性，最低抑制浓度为585μgmL-1。二维薄层色谱分析揭示了负责抗氧化活性的特定氨基酸，分子量截留超滤表明，<2-kDa馏分具有最高的抗氧化活性，而IC50值最低（0.43和0.22 mg mL -1分别表示DPPH和ABTS的抗氧化活性）。这是尼罗河鲈鱼废水作为天然抗氧化剂和营养成分被再利用的第一份报告。
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