Induction of viable but non-culturable (VBNC) state in Listeria monocytogenes by Origanum vulgare L. (OVEO) and Rosmarinus officinalis L. essential oils (ROEO) in a meat-based broth and phosphate-buffered saline was investigated. Occurrence of culturability loss on brain-heart infusion agar in L. monocytogenes cells (approximately 7 log CFU/mL) exposed to OVEO (5 and 2.5 μL/mL) or ROEO (10 and 5 μL/mL) individually in meat broth and phosphate-buffered saline, and their ability to restore culturability during a recovery treatment in phosphate-buffered saline + glucose (6 or 24 h, 30 °C) was evaluated. Viability of cells exposed to OVEO and ROEO was investigated with measurements of damage on integrity and membrane potential and enzymatic, efflux pump and respiratory activities. L. monocytogenes lost culturability after a 60 or 180 min-exposure to OVEO (5 and 2.5 μL/mL) or ROEO (10 and 5 μL/mL) in phosphate-buffered saline and meat broth. Cells exposed to ROEO in meat broth (5 and 10 μL/mL) and phosphate-buffered saline (5 μL/mL) restored culturability after 24 h of recovery treatment. Cells with lost culturability had variable subpopulation sizes (10.8–98.5%) with damage on measured physiological functions. Cells with lost culturability induced by OVEO or ROEO had improvements in physiological functions during recovery treatment, being indicative of viability. These results indicate that OVEO and ROEO could cause VBNC state in L. monocytogenes.

研究了在肉汤和磷酸盐缓冲液中，牛至属植物（OVEO）和迷迭香（Rosmarinus officinalis L.）精油（ROEO）在单核细胞增生李斯特氏菌中诱导了存活但不可培养的状态。在脑心脏浸液琼脂培养性损失的发生L.菌 肉汤和磷酸盐缓冲液中分别暴露于OVEO（5和2.5μL/ mL）或ROEO（10和5μL/ mL）的细胞（约7 log CFU / mL）及其在恢复治疗期间恢复可培养性的能力评估磷酸盐缓冲液中的葡萄糖+葡萄糖（6或24小时，30°C）。通过测量完整性和膜电位以及酶，外排泵和呼吸活动的损伤，研究了暴露于OVEO和ROEO的细胞的活力。单核细胞增生李斯特菌在磷酸盐缓冲液和肉汤中暴露于OVEO（5和2.5μL/ mL）或ROEO（10和5μL/ mL）60或180分钟后，失去了可培养性。肉汤（5和10μL/ mL）和磷酸盐缓冲液（5μL/ mL）中暴露于ROEO的细胞在恢复处理24小时后恢复了可培养性。具有丧失可培养性的细胞具有可变的亚群大小（10.8–98.5％），对测量的生理功能有损害。由OVEO或ROEO诱导的丧失可培养性的细胞在恢复治疗期间的生理功能有所改善，这表明其具有活力。这些结果表明OVEO和ROEO可能导致单核细胞增生李斯特菌的VBNC状态。