The checkpoint molecule human leukocyte antigen (HLA)-G has restricted tissue expression, and plays a role in the establishment of maternal tolerance to the semi-allogenic fetus during pregnancy by expression on the trophoblast cells in the placenta. HLA-G exists in at least seven well-described mRNA isoforms, of which four are membrane-bound and three soluble. Regulation of the tissue expression of HLA-G and its isoforms is relatively unknown. Therefore, it is important to understand the regulation of HLA-G, and the HLA-G+ choriocarcinoma cell line JEG-3 is a widely used cellular model. We hypothesized that cytokines present in the microenvironment can regulate the HLA-G expression profile. In the present study, we systematically stimulated JEG-3 cells with various concentrations of IL-2, IL-4 IL-6, IL-10, IL-12, IL-15, IL-17A, TGF-β1, TNF-α and IFN-γ1b. The results suggest that IFN-γ plays a role in maintenance of HLA-G expression, while IL-10 might be involved in regulation of the isoform profile.

中文翻译：

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绒毛膜癌细胞株JEG-3的细胞因子刺激导致HLA-G表达谱的改变。

检查点分子人类白细胞抗原（HLA）-G具有受限的组织表达，并通过在胎盘中的滋养层细胞上表达而在孕期建立对半同种异体胎儿的母体耐受中发挥作用。HLA-G存在于至少七个众所周知的mRNA亚型中，其中四个是膜结合的，三个是可溶的。HLA-G及其同工型的组织表达的调控相对未知。因此，重要的是要了解HLA-G的调控，并且HLA-G +绒癌细胞系JEG-3是广泛使用的细胞模型。我们假设存在于微环境中的细胞因子可以调节HLA-G表达谱。在本研究中，我们系统地刺激了各种浓度的IL-2，IL-4，IL-6，IL-10，IL-12，IL-15，IL-17A，TGF-β1，JEG-3细胞，TNF-α和IFN-γ1b。结果表明，IFN-γ在维持HLA-G表达中起作用，而IL-10可能参与同种型谱的调节。