当前位置：
X-MOL 学术
›
J. Proteomics
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Impact of Staphylococcus aureus infection on the late lactation goat milk proteome: New perspectives for monitoring and understanding mastitis in dairy goats.
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-04-08 , DOI: 10.1016/j.jprot.2020.103763 Salvatore Pisanu 1 , Carla Cacciotto 1 , Daniela Pagnozzi 1 , Sergio Uzzau 2 , Claudia Pollera 3 , Martina Penati 3 , Valerio Bronzo 3 , Maria Filippa Addis 4
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-04-08 , DOI: 10.1016/j.jprot.2020.103763 Salvatore Pisanu 1 , Carla Cacciotto 1 , Daniela Pagnozzi 1 , Sergio Uzzau 2 , Claudia Pollera 3 , Martina Penati 3 , Valerio Bronzo 3 , Maria Filippa Addis 4
Affiliation
|
The milk somatic cell count (SCC) is a standard parameter for monitoring intramammary infections (IMI) in dairy ruminants. In goats, however, the physiological increase in SCC occurring in late lactation heavily compromises its reliability. To identify and understand milk protein changes specifically related to IMI, we carried out a shotgun proteomics study comparing high SCC late lactation milk from goats with subclinical Staphylococcus aureus IMI and from healthy goats to low SCC mid-lactation milk from healthy goats. As a result, we detected 52 and 19 differential proteins (DPs) in S. aureus-infected and uninfected late lactation milk, respectively. Unexpectedly, one of the proteins higher in uninfected milk was serum amyloid A. On the other hand, 38 DPs were increased only in S. aureus-infected milk and included haptoglobin and numerous cytoskeletal proteins. Based on STRING analysis, the DPs unique to S. aureus infected milk were mainly involved in defense response, cytoskeleton organization, cell-to-cell, and cell-to-matrix interactions. Being tightly and specifically related to infectious/inflammatory processes, these proteins may hold promise as more reliable markers of IMI than SCC in late lactation goats. SIGNIFICANCE: The biological relevance of our results lies in the increased understanding of the changes specifically related to bacterial infection of the goat udder in late lactation. The DPs present only in S. aureus infected milk may find application as markers for improving the specificity of subclinical mastitis monitoring and detection in dairy goats in late lactation, when other widespread tools such as the SCC lose diagnostic value.
中文翻译：
金黄色葡萄球菌感染对泌乳后期山羊奶蛋白质组的影响：监测和了解奶山羊乳腺炎的新观点。
牛奶体细胞计数（SCC）是用于监测奶牛反刍动物乳房内感染（IMI）的标准参数。但是，在山羊中，泌乳后期发生的SCC生理增加严重损害了其可靠性。为了识别和了解与IMI特别相关的牛奶蛋白质变化，我们进行了shot弹枪蛋白质组学研究，比较了具有亚临床金黄色葡萄球菌IMI的山羊和健康山羊的高SCC晚期泌乳牛奶与健康山羊的低SCC中期泌乳。结果，我们在金黄色葡萄球菌感染的和未感染的晚期泌乳乳中分别检测到52和19个差异蛋白（DPs）。出乎意料的是，未感染的牛奶中蛋白质含量较高的一种蛋白质是血清淀粉样蛋白A。另一方面，仅S蛋白质增加了38 DPs。金黄色葡萄球菌感染的牛奶，包括触珠蛋白和许多细胞骨架蛋白。根据STRING分析，金黄色葡萄球菌感染的牛奶特有的DP主要参与防御反应，细胞骨架组织，细胞间和细胞间相互作用。这些蛋白与感染/炎症过程密切相关，并且与感染/炎症过程密切相关，在晚期哺乳山羊中，它们可能是比SCC更可靠的IMI标志物。意义：我们研究结果的生物学相关性在于人们对后期泌乳中与山羊乳房细菌感染特别相关的变化的了解增加了。仅存在于金黄色葡萄球菌感染的牛奶中的DPs可以作为标记物用于提高泌乳后期泌乳山羊亚临床乳腺炎监测和检测的特异性，
更新日期：2020-04-08
中文翻译：
金黄色葡萄球菌感染对泌乳后期山羊奶蛋白质组的影响：监测和了解奶山羊乳腺炎的新观点。
牛奶体细胞计数（SCC）是用于监测奶牛反刍动物乳房内感染（IMI）的标准参数。但是，在山羊中，泌乳后期发生的SCC生理增加严重损害了其可靠性。为了识别和了解与IMI特别相关的牛奶蛋白质变化，我们进行了shot弹枪蛋白质组学研究，比较了具有亚临床金黄色葡萄球菌IMI的山羊和健康山羊的高SCC晚期泌乳牛奶与健康山羊的低SCC中期泌乳。结果，我们在金黄色葡萄球菌感染的和未感染的晚期泌乳乳中分别检测到52和19个差异蛋白（DPs）。出乎意料的是，未感染的牛奶中蛋白质含量较高的一种蛋白质是血清淀粉样蛋白A。另一方面，仅S蛋白质增加了38 DPs。金黄色葡萄球菌感染的牛奶，包括触珠蛋白和许多细胞骨架蛋白。根据STRING分析，金黄色葡萄球菌感染的牛奶特有的DP主要参与防御反应，细胞骨架组织，细胞间和细胞间相互作用。这些蛋白与感染/炎症过程密切相关，并且与感染/炎症过程密切相关，在晚期哺乳山羊中，它们可能是比SCC更可靠的IMI标志物。意义：我们研究结果的生物学相关性在于人们对后期泌乳中与山羊乳房细菌感染特别相关的变化的了解增加了。仅存在于金黄色葡萄球菌感染的牛奶中的DPs可以作为标记物用于提高泌乳后期泌乳山羊亚临床乳腺炎监测和检测的特异性，
京公网安备 11010802027423号