Coumarins are important especialized metabolites that have potential application in different fields. For the purpose of obtaining fractions enriched with coumarins, extracts from the leaves and inflorescences of Pterocaulon lorentzii Malme (Asteraceae) were obtained by different methods and solvents, i.e. with water at 60 °C, with dichloromethane at room temperature (25 °C) and by means of supercritical CO₂ (SC-CO₂) extraction at 40 °C with gradual pressure increments (90, 120, 150, 200 and 300 bar). The coumarins content in the extracts was calculated using ultra-fast liquid chromatography analyses, which allowed the characterization of the coumarins. Sabandinol (5-(2′,3′-dihydroxy-3′-methylbutyloxy)-6,7-methylenedioxycoumarin) was the main coumarin found in the aqueous and dichlorometane extracts. The epoxy-derivative 5-(2′,3′-epoxy-3′-methylbutyloxy)-6,7-methylenedioxycoumarin was identified as the major component of all extracts obtained by SC-CO₂. The SC-CO₂ extract from the inflorescences proved to be richer in coumarins than the extract from the leaves. The results suggest that when the objective is to obtain epoxy-coumarins, the method of choice should be SC-CO₂, as this technique was effective for preventing the ring-opening of the epoxy group.

香豆素是重要的专业化代谢物，在不同领域都有潜在的应用。用于获得从叶和花序与香豆素富集级分，提取物的目的翼茎草属lorentzii MALME（菊科）通过与水不同的方法和溶剂，即在60℃下在室温（25℃）和得到的，用二氯甲烷通过超临界CO ₂（SC-CO ₂）在40°C下以逐渐增加的压力（90、120、150、200和300 bar）萃取。使用超快速液相色谱分析法计算提取物中的香豆素含量，从而可以鉴定香豆素。萨班地诺（5-（2'，3'-二羟基-3'-甲基丁氧基）-6,7-亚甲基二氧基香豆素）是在水性和二氯甲烷提取物中发现的主要香豆素。环氧衍生物5-（2'，3'-环氧-3'-甲基丁氧基）-6,7-亚甲基二氧基香豆素被确定为通过SC-CO ₂获得的所有提取物的主要成分。来自花序的SC-CO ₂提取物比来自叶的提取物富含香豆素。结果表明，当目标是获得环氧香豆素时，选择的方法应该是SC-CO ₂，因为该技术对于防止环氧基的开环是有效的。