One of the most important diseases of barley (Hordeum vulgare) is powdery mildew, caused by Blumeria graminis f. sp. hordei. Spring barley line 173‐1‐2 was selected from a Moroccan landrace and revealed broad‐spectrum resistance to powdery mildew. The objective of this study was to map and characterize the gene for seedling powdery mildew resistance in this line. After crossing with the susceptible cultivar ‘Manchuria’, genetic analysis of F₂ and F₃ families at the seedling stage revealed powdery mildew resistance in line 173‐1‐2 conditioned by a single recessive gene. Molecular analysis of non‐segregating homozygous resistant and homozygous susceptible F₂ plants conducted on the DArTseq platform (Diversity Arrays Technology Pty Ltd) identified significant markers which were converted to allele‐specific PCR markers and tested among 94 F₂ individuals. The new resistance gene was mapped on the long arm of chromosome 6H. No other powdery mildew recessive resistance gene has been located on 6H so far. Therefore, we concluded that the 173‐1‐2 barley line carries a novel recessive resistance gene designated as mlmr.

中文翻译：

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大麦（Hordeum vulgare）抗白粉病（Blumeria graminis f。sp。hordei）的新型隐性基因的鉴定。

大麦（Hordeum vulgare）最重要的疾病之一是白粉病，它是由Blumeria graminis f引起的。sp。大麦。春季大麦品系173-1-2是从摩洛哥地方品种中选出的，显示出广谱的抗白粉病性。这项研究的目的是在该品系中定位和鉴定抗白粉病基因。与易感品种'Manchuria'杂交后，对苗期F ₂和F ₃家族的遗传分析显示，由单一隐性基因调节的品系1731-2的白粉病抗性。非分离纯合子抗性和纯合子易感F _2的分子分析在DArTseq平台（Diversity Arrays Technology Pty Ltd）上进行的植物鉴定了重要的标记，这些标记被转化为等位基因特异性PCR标记，并在94个F ₂个体中进行了测试。新的抗性基因被定位在6H染色体的长臂上。到目前为止，在6H上还没有发现其他的白粉病隐性抗性基因。因此，我们得出的结论是173-1-2大麦系带有一个新的隐性抗性基因，称为mlmr。