Hybrid giant lipid vesicles incorporating a PMMA-based copolymer,Biochimica et Biophysica Acta (BBA) - General Subjects

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Hybrid giant lipid vesicles incorporating a PMMA-based copolymer
Biochimica et Biophysica Acta (BBA) - General Subjects ( IF 2.8 ) Pub Date : 2020-04-07 , DOI: 10.1016/j.bbagen.2020.129611
Ylenia Miele ₁ , Anne-Françoise Mingotaud ₂ , Enrico Caruso ₃ , Miryam C Malacarne ₃ , Lorella Izzo ₃ , Barbara Lonetti ₂ , Federico Rossi ₄

Affiliation

Background

In recent years, there has been a growing interest in the formation of copolymer-lipid hybrid self-assemblies, which allow combining and improving the main features of pure lipid-based and copolymer-based systems known for their potential applications in the biomedical field. As the most common method used to obtain giant vesicles is electroformation, most systems so far used low T_g polymers for their flexibility at room temperature.

Methods

Copolymers used in the hybrid vesicles have been synthesized by a modified version of the ATRP, namely the Activators ReGenerated by Electron Transfer ATRP and characterized by NMR and DSC. Giant hybrid vesicles have been obtained using electroformation and droplet transfer method. Confocal fluorescence microscopy was used to image the vesicles.

Results

Electroformation enabled to obtain hybrid vesicles in a narrow range of compositions (15 mol% was the maximum copolymer content). This range could be extended by the use of a droplet transfer method, which enabled obtaining hybrid vesicles incorporating a methacrylate-based polymer in a wide range of compositions. Proof of the hybrid composition was obtained by fluorescence microscopy using labeled lipids and copolymers.

Conclusions

This work describes for the first time, to the best of our knowledge, the formation of giant hybrid polymer/lipid vesicles formed with such a content of a polymethylmethacrylate copolymer, the glass temperature of which is above room temperature.

General significance

This work shows that polymer structures, more complex than the ones mostly employed, can be possibly included in giant hybrid vesicles by using the droplet transfer method. This will give easier access to functionalized and stimuli-responsive giant vesicles and to systems exhibiting a tunable permeability, these systems being relevant for biological and technological applications.

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