Bone morphogenetic protein signaling regulates Id1 mediated neural stem cell quiescence in the adult zebrafish brain via a phylogenetically conserved enhancer module,STEM CELLS

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Bone morphogenetic protein signaling regulates Id1 mediated neural stem cell quiescence in the adult zebrafish brain via a phylogenetically conserved enhancer module
STEM CELLS ( IF 4.0 ) Pub Date : 2020-04-11 , DOI: 10.1002/stem.3182
Gaoqun Zhang ₁ , Marco Ferg ₁ , Luisa Lübke ₁ , Masanari Takamiya ₁ , Tanja Beil ₁ , Victor Gourain ₁ , Nicolas Diotel ₂ , Uwe Strähle ₁ , Sepand Rastegar ₁

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In the telencephalon of adult zebrafish, the inhibitor of DNA binding 1 (id1) gene is expressed in radial glial cells (RGCs), behaving as neural stem cells (NSCs), during constitutive and regenerative neurogenesis. Id1 controls the balance between resting and proliferating states of RGCs by promoting quiescence. Here, we identified a phylogenetically conserved cis‐regulatory module (CRM) mediating the specific expression of id1 in RGCs. Systematic deletion mapping and mutation of conserved transcription factor binding sites in stable transgenic zebrafish lines reveal that this CRM operates via conserved smad1/5 and 4 binding motifs under both homeostatic and regenerative conditions. Transcriptome analysis of injured and uninjured telencephala as well as pharmacological inhibition experiments identify a crucial role of bone morphogenetic protein (BMP) signaling for the function of the CRM. Our data highlight that BMP signals control id1 expression and thus NSC proliferation during constitutive and induced neurogenesis.

中文翻译：

骨形态发生蛋白信号通过系统发育保守的增强子模块调节成年斑马鱼大脑中 Id1 介导的神经干细胞静止

在成年斑马鱼的端脑中，DNA 结合 1 (id1) 基因的抑制剂在放射状胶质细胞 (RGC) 中表达，在组成和再生神经发生过程中表现为神经干细胞 (NSC)。Id1 通过促进静止来控制 RGC 的静止和增殖状态之间的平衡。在这里，我们确定了一个系统发育保守的顺式调节模块 (CRM)，它介导 id1 在 RGC 中的特异性表达。稳定的转基因斑马鱼系中保守转录因子结合位点的系统缺失作图和突变表明，这种 CRM 在稳态和再生条件下通过保守的 smad1/5 和 4 结合基序进行操作。受伤和未受伤端脑的转录组分析以及药理抑制实验确定了骨形态发生蛋白 (BMP) 信号传导对 CRM 功能的关键作用。我们的数据强调 BMP 信号控制 id1 表达，从而在组成性和诱导神经发生期间控制 NSC 增殖。

更新日期：2020-04-11

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