We aimed to evaluate the chemical and behavioral effects of 2,5-dihydro-2,4,5-trimethylthiazoline (TMT) after olfactory exposure and to verify their influence in the expression of acute audiogenic seizures in the Wistar Audiogenic Rat (WAR) strain. PROTOCOL 1: TMT gas chromatography was applied to define odor saturation in a chamber to different concentrations, time required for saturation and desaturation, and if saturation was homogeneous. Also, male Adult Wistar rats were exposed to saline (SAL) or to different TMT concentrations and their behaviors were evaluated (neuroethology). PROTOCOL 2: Male adult WARs were exposed for 15 s to SAL or TMT, followed by sound stimulation for 1 min or until tonic–clonic convulsion. Behavioral analysis included latencies (wild running and tonic–clonic convulsion), seizure severity indexes, and neuroethology. Gas chromatography established a saturation homogeneous to different concentrations of TMT, indicating that saturation and desaturation occurred in 30 min. TMT triggered fear-like or aversion-like reactions associated with reduction in motor activity and in grooming behavior, in the 2 highest concentrations. Pure TMT presented anticonvulsant properties, such as less-severe seizure phenotype, as well as a decrease in tonic–clonic convulsion expression. TMT elicited fear-like or aversion-like behaviors in Wistar and WAR and can be utilized in a quantifiable and controllable way. Our results suggested possible antagonism between “fear-related” or “aversion-related” and “seizure-related” networks.

中文翻译：

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捕食者气味（TMT）诱导的大鼠行为改变是否能够抑制癫痫发作？

我们旨在评估嗅觉暴露后2,5-二氢-2,4,5-三甲基噻唑啉（TMT）的化学和行为效应，并验证其在Wistar音频成年大鼠（WAR）株中急性音频性发作的表达中的影响。方案1：使用TMT气相色谱法将室内的气味饱和度定义为不同的浓度，饱和度和去饱和度所需的时间以及饱和度是否均匀。另外，将雄性成年Wistar大鼠暴露于生理盐水（SAL）或不同的TMT浓度，并评估其行为（神经行为学）。协议2：男性成年战争s暴露于SAL或TMT中15 s，然后进行声音刺激1分钟或直至强直-阵挛性抽搐。行为分析包括潜伏期（狂奔和强直-阵挛性抽搐），癫痫发作严重程度指数和神经行为学。气相色谱法建立了对不同浓度的TMT均一的饱和度，表明在30分钟内发生了饱和和去饱和。在2种最高浓度下，TMT引发与恐惧活动或类似厌恶反应有关的运动活动和修饰行为减少。纯TMT表现出抗惊厥特性，例如较轻的癫痫发作表型，以及强直-阵挛性惊厥表达降低。TMT引发恐惧感或厌恶感Wistar和WAR中的行为，并且可以以可量化和可控制的方式加以利用。我们的结果表明“恐惧相关”或“厌恶相关”网络与“癫痫相关”网络之间可能存在对抗。