Mature landfill leachate (MLL) tend to be highly contaminated due to the presence of refractory contaminants such as humic–fulvic substances, xenobiotic compounds, and heavy metals. This study investigated the treatment efficiency of MLL by deploying Cladosporium sp., Trichoderma asperellum, and Tyromyces chioneus fungal strains. Chemical oxygen demand (COD) and soluble COD (sCOD) removal efficiencies were assessed along with the evaluation of lignocellulosic enzymatic activities of laccase (Lac), lignin-peroxidase (LiP), and manganese-peroxidase (MnP). Glucose, woodchips, and wheat straw were utilized as co-substrates. Higher percentage of COD and sCOD reduction efficiencies and lignocellulosic enzymatic activities were found for woodchips than glucose and wheat-straw. The highest sCOD removal rates were 44%, 38% and 59% by Cladosporium sp., T. asperellum, and T. chioneus, respectively. Overall, Lac activity was significantly higher than LiP and MnP activity for all three species. Tyromyces chioneus was the most effective strain among the three selected fungi in terms of COD and sCOD reduction efficiencies and high enzymatic activities of 165, 14 and 20 U/L were detected for Lac, LiP, and MnP, respectively. Tyromyces chioneus is a potentially effective fungal strain for the enhanced bioremediation of MLL and its further investigation is recommended to explore the removal of recalcitrant contaminants from problematic wastewater.

中文翻译：

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利用木片和麦秸作为共同基质对成熟的垃圾渗滤液进行真菌处理。

成熟的垃圾渗滤液（MLL）由于存在腐殖质-富夫菌物质，异种生物化合物和重金属等难熔污染物而容易受到高度污染。这项研究调查了通过部署Cladosporium sp。，Trichoderma asperellum和Tyromyces chioneus对MLL的治疗效果。真菌菌株。评估了化学需氧量（COD）和可溶性COD（sCOD）去除效率，并评估了漆酶（Lac），木质素过氧化物酶（LiP）和锰过氧化物酶（MnP）的木质纤维素酶活性。葡萄糖，木片和麦秸被用作共同的底物。发现木片的COD和sCOD降低效率和木质纤维素酶活性的百分比高于葡萄糖和麦秸。Cladosporium sp。，T。asperellum和T. chioneus的最高sCOD去除率分别为44％，38％和59％。总体而言，这三个物种的Lac活性均显着高于LiP和MnP活性。斑节菌就降低COD和sCOD的效率而言，这是三种所选真菌中最有效的菌株，分别检测到Lac，LiP和MnP的高酶活性分别为165、14和20 U / L。Tyromyces chioneus是一种潜在的有效真菌菌株，可增强MLL的生物修复作用，建议对其进行进一步研究，以探索从有问题的废水中去除顽固性污染物的方法。