Soybean mosaic virus (SMV) is one of the most destructive viral diseases in soybean and causes severe reduction of soybean yield and destroys the seed quality. However, the production of SMV resistant plants by transgenic is the most effective and economical means. Based on our previous yeast two-hybrid assay, the GmVma12 was selected as a strong candidate gene for further function characterization. Here we transformed soybean plants with a construct containing inverted repeat of-GmVma12 sequence to analyze the role of GmVma12 during SMV invasion. Totals of 33 T₀ and 160 T₁ plants were confirmed as positive transgenic plants through herbicide application, PCR detection and LibertyLink^® strip screening. Based on the segregation ratio and Southern Blot data, T₁ lines No. 3 and No. 7 were selected to generate T₂ plants. After SMV-SC15 inoculation, 41 T₁ and 38 T₂ plants were identified as highly resistant, and their quantification disease levels were much lower than non-transformed plants. The transcript level of GmVma12 in T₂ plants decreased to 70% of non-transformed plants. The expression level of SMV-CP transcript in T₂ transgenic plants was lower than that in non-transformed plants and SMV CP protein in T₂ plants could not be detected by Enzyme-linked Immunosorbent assay, which indicated that SMV production would be inhibited in transgenic plants. Moreover, coat mottles of T₂ seeds were obliterated significantly. In conclusion, inverted repeat of the hairpin structure of GmVma12 interfered with the transcription of GmVma12, which can induce resistance to SMV in soybean. This research lays the foundation for the mechanism of SMV pathogenesis, and provides new ideas for SMV prevention and control.

大豆花叶病毒（SMV）是大豆中最具破坏性的病毒性疾病之一，会严重降低大豆产量并破坏种子质量。然而，通过转基因生产抗SMV的植物是最有效和经济的手段。基于我们之前的酵母双杂交检测，GmVma12被选作进一步功能表征的强候选基因。在这里，我们用含有反向重复的GmVma12序列的构建体转化了大豆植株，以分析GmVma12在SMV入侵过程中的作用。33的T总计₀和160种₁植物被证实为通过施用除草剂，PCR检测和LibertyLink正转基因植物^®带状筛选。基于分离率和Southern Blot数据，选择3号和₁号T ₁系产生T ₂植株。接种SMV-SC15后，鉴定出41株T ₁和38株T ₂具有高度抗性，其定量病害水平大大低于未转化的植物。T ₂植物中GmVma12的转录水平降至未转化植物的70％。在T ₂转基因植物中SMV- CP转录物的表达水平低于未转化植物，而在S _2中SMV CP蛋白的表达水平较低。酶联免疫吸附法无法检测到植物，这表明转基因植物中SMV的产生将受到抑制。而且，T ₂种子的被毛斑被显着消除。最后，的发夹结构的反向重复GmVma12干扰的转录GmVma12，可诱导抗SMV大豆。该研究为SMV发病机理奠定了基础，为SMV的预防和控制提供了新思路。