The aim of the study was to determine the expression profiles of GABAA, GABAB, and GAT1 using RT-PCR and the immunoreactivity of GAT1 via immunohistochemical and immunofluorescence assays in CDV-infected brain tissue of dogs. For this purpose, dogs with CDV and dogs without CDV were selected. The mRNA transcript levels of GABAA, GABAB, and GAT1 were significantly downregulated in brain tissue in the CDV-infected group as compared with that in non-CDV-infected brain tissue in the control group (p < 0.01, p < 0.001). In addition, the immunoreactivity of GAT1 in CDV-infected brain tissue was significantly lower than in the uninfected group (p < 0.05). We conclude that one of the main causes of myoclonus in CDV infections may be the blockage of postsynaptic inhibition in neurons or a lack of metabolism of GABA. In addition, a GABA neurotransmission imbalance could play a role in demyelination in CDV infections.

中文翻译：

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犬瘟热病毒诱导狗脑组织中GABAA，GABAB和GAT1表达的下调。

该研究的目的是通过RT-PCR来确定GABAA，GABAB和GAT1的表达谱，并通过免疫组化和免疫荧光测定法确定CDV感染的狗脑组织中GAT1的免疫反应性。为此，选择了具有CDV的狗和没有CDV的狗。与对照组相比，CDV感染组脑组织中GABAA，GABAB和GAT1的mRNA转录水平显着下调（p <0.01，p <0.001）。此外，GAT1在CDV感染的脑组织中的免疫反应性显着低于未感染组（p <0.05）。我们得出结论，CDV感染中肌阵挛的主要原因之一可能是神经元中突触后抑制的阻滞或GABA代谢不足。此外，